Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12861-015-0058-9
Title: Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy
Authors: De Silva, D
Kunasegaran, K 
Ghosh, S 
Pietersen, A.M 
Keywords: hormone
transcriptome
animal
breast tumor
cell proliferation
cytology
female
genetics
metabolism
mouse
pathology
pregnancy
udder
Animals
Breast Neoplasms
Cell Proliferation
Female
Hormones
Mammary Glands, Animal
Mice
Pregnancy
Transcriptome
Issue Date: 2015
Publisher: BioMed Central Ltd.
Citation: De Silva, D, Kunasegaran, K, Ghosh, S, Pietersen, A.M (2015). Transcriptome analysis of the hormone-sensing cells in mammary epithelial reveals dynamic changes in early pregnancy. BMC Developmental Biology 15 (1) : 7. ScholarBank@NUS Repository. https://doi.org/10.1186/s12861-015-0058-9
Abstract: Background: Alveoli, the milk-producing units of the mammary gland, are generated during pregnancy by collaboration of different epithelial cell types. We present the first analysis of transcriptional changes within the hormone sensing population during pregnancy. Hormone-receptor positive (HR+) cells play a key role in the initiation of alveologenesis as they sense systemic hormonal changes and translate these into local instructions for neighboring HR- cells. We recently showed that IGF2 is produced specifically by HR+ cells in early pregnancy, but is undetectable in the virgin state. Here, we define the transcriptome of HR+ cells in early pregnancy with the aim to elucidate additional changes that are unique for this dynamic developmental time window. Results: We harvested mammary glands from virgin, 3-day and 7-day pregnant mice and isolated a few hundred hormone-sensing cells per animal by FACS for microarray analysis. There was a high concordance between animals with a clear induction of cell cycle progression genes at day 3 of pregnancy and molecules involved in paracrine signalling at day 7. Conclusions: These findings underscore the proliferative capacity of HR+ cells upon specific stimuli and elucidate developmentally-restricted changes in cellular communication. Since the majority of breast cancers are HR+, with a variable proportion of HR+ cells per tumor, we anticipate that this data set will aid further studies into the regulation of HR+ cell proliferation and the role of heterotypic signalling within tumors. © 2015 De Silva et al.
Source Title: BMC Developmental Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/174289
ISSN: 1471213X
DOI: 10.1186/s12861-015-0058-9
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