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https://doi.org/10.1038/s41467-018-04211-x
Title: | Multidimensional heuristic process for high-yield production of astaxanthin and fragrance molecules in Escherichia coli | Authors: | Zhang, C Seow, V.Y Chen, X Too, H.-P |
Keywords: | astaxanthin beta carotene enzyme variant fragrance linalool lycopene nerolidol zeaxanthin astaxanthin beta carotene Escherichia coli protein fragrance lycopene xanthophyll biochemical composition chemical binding enzyme activity gene expression genetic analysis multivariate analysis optimization Article binding site biosynthesis drug manufacture drug screening enzyme kinetics Escherichia coli molecular library multidimensional heuristic process nonhuman process optimization promoter region ribosome signal transduction chemistry Escherichia coli evaluation study genetics metabolic engineering metabolism procedures Escherichia coli beta Carotene Escherichia coli Escherichia coli Proteins Lycopene Metabolic Engineering Metabolic Networks and Pathways Odorants Xanthophylls |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Zhang, C, Seow, V.Y, Chen, X, Too, H.-P (2018). Multidimensional heuristic process for high-yield production of astaxanthin and fragrance molecules in Escherichia coli. Nature Communications 9 (1) : 1858. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-04211-x | Abstract: | Optimization of metabolic pathways consisting of large number of genes is challenging. Multivariate modular methods (MMMs) are currently available solutions, in which reduced regulatory complexities are achieved by grouping multiple genes into modules. However, these methods work well for balancing the inter-modules but not intra-modules. In addition, application of MMMs to the 15-step heterologous route of astaxanthin biosynthesis has met with limited success. Here, we expand the solution space of MMMs and develop a multidimensional heuristic process (MHP). MHP can simultaneously balance different modules by varying promoter strength and coordinating intra-module activities by using ribosome binding sites (RBSs) and enzyme variants. Consequently, MHP increases enantiopure 3S,3?S-astaxanthin production to 184 mg l-1 day-1 or 320 mg l-1. Similarly, MHP improves the yields of nerolidol and linalool. MHP may be useful for optimizing other complex biochemical pathways. © 2018 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174222 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-018-04211-x |
Appears in Collections: | Elements Staff Publications |
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