Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-07149-2
Title: Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset
Authors: Priti, A 
Ong, H.T 
Toyama, Y 
Padmanabhan, A 
Dasgupta, S 
Krajnc, M
Zaidel-Bar, R 
Keywords: myosin adenosine triphosphatase
Caenorhabditis elegans protein
myosin
myosin adenosine triphosphatase
amino acid
fertility
germ cell
laser
membrane
nematode
Article
Caenorhabditis elegans
cytoskeleton
germ line
gonad
laser surgery
mathematical model
microsurgery
nonhuman
structure analysis
surface property
tension
animal
biological model
cytoplasmic streaming
germ cell
giant cell
metabolism
Caenorhabditis elegans
Actomyosin
Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Cytoplasmic Streaming
Germ Cells
Giant Cells
Gonads
Models, Biological
Myosins
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Priti, A, Ong, H.T, Toyama, Y, Padmanabhan, A, Dasgupta, S, Krajnc, M, Zaidel-Bar, R (2018). Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset. Nature Communications 9 (1) : 4694. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-07149-2
Abstract: Syncytial architecture is an evolutionarily-conserved feature of the germline of many species and plays a crucial role in their fertility. However, the mechanism supporting syncytial organization is largely unknown. Here, we identify a corset-like actomyosin structure within the syncytial germline of Caenorhabditis elegans, surrounding the common rachis. Using laser microsurgery, we demonstrate that actomyosin contractility within this structure generates tension both in the plane of the rachis surface and perpendicular to it, opposing membrane tension. Genetic and pharmacological perturbations, as well as mathematical modeling, reveal a balance of forces within the gonad and show how changing the tension within the actomyosin corset impinges on syncytial germline structure, leading, in extreme cases, to sterility. Thus, our work highlights a unique tissue-level cytoskeletal structure, and explains the critical role of actomyosin contractility in the preservation of a functional germline. © 2018, The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174197
ISSN: 2041-1723
DOI: 10.1038/s41467-018-07149-2
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