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Title: A Transcriptomic Signature of Mouse Liver Progenitor Cells
Authors: Passman, A.M
Low, J
London, R
Tirnitz-Parker, J.E.E
Miyajima, A
Tanaka, M
Strick-Marchand, H
Darlington, G.J
Finch-Edmondson, M 
Ochsner, S
Zhu, C
Whelan, J
Callus, B.A
Yeoh, G.C.T
Keywords: latent transforming growth factor beta binding protein
latent transforming growth factor beta binding protein 3
minichromosome maintenance protein 2
nerve cell adhesion molecule
nerve cell adhesion molecule 1
pyruvate kinase
pyruvate kinase isozyme M2
unclassified drug
animal cell
animal experiment
animal model
animal tissue
cell proliferation
controlled study
embryonic stem cell
enzyme activation
gene cluster
limit of quantitation
liver carcinogenesis
liver cell carcinoma
liver progenitor cell
microarray analysis
muscle stem cell
protein expression
stem cell
Issue Date: 2016
Citation: Passman, A.M, Low, J, London, R, Tirnitz-Parker, J.E.E, Miyajima, A, Tanaka, M, Strick-Marchand, H, Darlington, G.J, Finch-Edmondson, M, Ochsner, S, Zhu, C, Whelan, J, Callus, B.A, Yeoh, G.C.T (2016). A Transcriptomic Signature of Mouse Liver Progenitor Cells. Stem Cells International 2016 : 5702873. ScholarBank@NUS Repository.
Abstract: Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver. © 2016 Adam M. Passman et al.
Source Title: Stem Cells International
ISSN: 16879678
DOI: 10.1155/2016/5702873
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