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Title: Aberrant Splicing Promotes Proteasomal Degradation of L-type Ca v 1.2 Calcium Channels by Competitive Binding for CaV β Subunits in Cardiac Hypertrophy
Authors: Hu, Z 
Wang, J.-W 
Yu, D 
Soon, J.L 
De Kleijn, D.P.V 
Foo, R 
Liao, P 
Colecraft, H.M
Soong, T.W 
Keywords: calcium channel L type
L-type calcium channel alpha(1C)
alternative RNA splicing
binding competition
protein degradation
RNA splicing
Alternative Splicing
Binding, Competitive
Calcium Channels, L-Type
Proteasome Endopeptidase Complex
RNA Splicing
Issue Date: 2016
Citation: Hu, Z, Wang, J.-W, Yu, D, Soon, J.L, De Kleijn, D.P.V, Foo, R, Liao, P, Colecraft, H.M, Soong, T.W (2016). Aberrant Splicing Promotes Proteasomal Degradation of L-type Ca v 1.2 Calcium Channels by Competitive Binding for CaV β Subunits in Cardiac Hypertrophy. Scientific Reports 6 : 35247. ScholarBank@NUS Repository.
Abstract: Decreased expression and activity of Ca V1.2 calcium channels has been reported in pressure overload-induced cardiac hypertrophy and heart failure. However, the underlying mechanisms remain unknown. Here we identified in rodents a splice variant of Ca V1.2 channel, named Ca V1.2 e21+22, that contained the pair of mutually exclusive exons 21 and 22. This variant was highly expressed in neonatal hearts. The abundance of this variant was gradually increased by 12.5-folds within 14 days of transverse aortic banding that induced cardiac hypertrophy in adult mouse hearts and was also elevated in left ventricles from patients with dilated cardiomyopathy. Although this variant did not conduct Ca 2+ ions, it reduced the cell-surface expression of wild-type Ca V1.2 channels and consequently decreased the whole-cell Ca 2+ influx via the Ca V1.2 channels. In addition, the Ca V1.2 e21+22 variant interacted with Ca V? subunits significantly more than wild-type Ca V1.2 channels, and competition of Ca V? subunits by Ca V1.2 e21+22 consequently enhanced ubiquitination and subsequent proteasomal degradation of the wild-type Ca V1.2 channels. Our findings show that the resurgence of a specific neonatal splice variant of Ca V1.2 channels in adult heart under stress may contribute to heart failure. © 2016 The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/srep35247
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