Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep40844
Title: Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas
Authors: Sun, Y
Wang, C
Wang, N
Jiang, X
Mao, H
Zhu, C
Wen, F
Wang, X
Lu, Z
Yue, G 
Xu, Z
Ye, J
Keywords: indoleacetic acid derivative
plant protein
protein binding
transcription factor
amino acid sequence
antagonists and inhibitors
Arabidopsis
chemistry
cytoskeleton
gene silencing
gene vector
genetics
growth, development and aging
Jatropha
metabolism
physiology
plant leaf
plant seed
plant virus
sequence alignment
signal transduction
transgenic plant
Amino Acid Sequence
Arabidopsis
Cytoskeleton
Gene Silencing
Genetic Vectors
Indoleacetic Acids
Jatropha
Plant Leaves
Plant Proteins
Plant Viruses
Plants, Genetically Modified
Protein Binding
Seeds
Sequence Alignment
Signal Transduction
Transcription Factors
Issue Date: 2017
Citation: Sun, Y, Wang, C, Wang, N, Jiang, X, Mao, H, Zhu, C, Wen, F, Wang, X, Lu, Z, Yue, G, Xu, Z, Ye, J (2017). Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas. Scientific Reports 7 : 40844. ScholarBank@NUS Repository. https://doi.org/10.1038/srep40844
Abstract: Seed size is a major determinant of seed yield but few is known about the genetics controlling of seed size in plants. Phytohormones cytokinin and brassinosteroid were known to be involved in the regulation of herbaceous plant seed development. Here we identified a homolog of Auxin Response Factor 19 (JcARF19) from a woody plant Jatropha curcas and genetically demonstrated its functions in controlling seed size and seed yield. Through Virus Induced Gene Silencing (VIGS), we found that JcARF19 was a positive upstream modulator in auxin signaling and may control plant organ size in J. curcas. Importantly, transgenic overexpression of JcARF19 significantly increased seed size and seed yield in plants Arabidopsis thaliana and J. curcas, indicating the importance of auxin pathway in seed yield controlling in dicot plants. Transcripts analysis indicated that ectopic expression of JcARF19 in J. curcas upregulated auxin responsive genes encoding essential regulators in cell differentiation and cytoskeletal dynamics of seed development. Our data suggested the potential of improving seed traits by precisely engineering auxin signaling in woody perennial plants.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/173947
ISSN: 20452322
DOI: 10.1038/srep40844
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