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https://doi.org/10.1016/j.antiviral.2017.12.003
Title: | A flavonoid compound library screen revealed potent antiviral activity of plant-derived flavonoids on human enterovirus A71 replication | Authors: | Min, Nyo Leong, Pok Thim Lee, Regina Ching Hua Khuan, Jeffery Seng Eng Chu, Justin Jang Hann |
Keywords: | Science & Technology Life Sciences & Biomedicine Pharmacology & Pharmacy Virology RIBOSOME ENTRY SITE MOUTH-DISEASE EPIDEMIOLOGY INFECTION EV71 MANAGEMENT SINGAPORE OUTBREAKS PROTEIN HAND |
Issue Date: | 1-Feb-2018 | Publisher: | ELSEVIER SCIENCE BV | Citation: | Min, Nyo, Leong, Pok Thim, Lee, Regina Ching Hua, Khuan, Jeffery Seng Eng, Chu, Justin Jang Hann (2018-02-01). A flavonoid compound library screen revealed potent antiviral activity of plant-derived flavonoids on human enterovirus A71 replication. ANTIVIRAL RESEARCH 150 : 60-68. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2017.12.003 | Abstract: | © 2017 Elsevier B.V. Hand Foot Mouth Disease (HFMD), resulting from human enterovirus A71 (HEVA71) infection can cause severe neurological complications leading to fatality in young children. Currently, there is no approved antiviral for therapeutic treatment against HEVA71 infection. In this study, a 500-compound flavonoid library was screened to identify potential inhibitors of HEVA71 using high-throughput immunofluorescence-based phenotypic screening method. Two lead flavonoid compounds, ST077124 and ST024734 at the non-cytotoxic concentration of 50 μM were found to be effective antivirals that inhibited replication of HEVA71, reducing infectious viral titers by 3.5 log10 PFU/ml and 2.5 log10 PFU/ml respectively. Our study revealed that ST077124 is a specific antiviral compound that inhibits human enteroviruses while ST024734 exhibited antiviral activity against human enteroviruses as well as dengue virus type-2. We also identified that both compounds affected the viral RNA transcription and translation machinery of HEVA71 but did not interfere with the viral internal ribosomal entry site (IRES) activity. Hence, our findings strongly suggest that ST077124 and ST024734 are effective antiviral compounds of minimal cytotoxicity and could serve as promising therapeutic agents against HEVA71 infection. | Source Title: | ANTIVIRAL RESEARCH | URI: | https://scholarbank.nus.edu.sg/handle/10635/173260 | ISSN: | 01663542 18729096 |
DOI: | 10.1016/j.antiviral.2017.12.003 |
Appears in Collections: | Staff Publications Elements |
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