Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2017.12.003
Title: A flavonoid compound library screen revealed potent antiviral activity of plant-derived flavonoids on human enterovirus A71 replication
Authors: Min, Nyo 
Leong, Pok Thim
Lee, Regina Ching Hua
Khuan, Jeffery Seng Eng
Chu, Justin Jang Hann 
Keywords: Science & Technology
Life Sciences & Biomedicine
Pharmacology & Pharmacy
Virology
RIBOSOME ENTRY SITE
MOUTH-DISEASE
EPIDEMIOLOGY
INFECTION
EV71
MANAGEMENT
SINGAPORE
OUTBREAKS
PROTEIN
HAND
Issue Date: 1-Feb-2018
Publisher: ELSEVIER SCIENCE BV
Citation: Min, Nyo, Leong, Pok Thim, Lee, Regina Ching Hua, Khuan, Jeffery Seng Eng, Chu, Justin Jang Hann (2018-02-01). A flavonoid compound library screen revealed potent antiviral activity of plant-derived flavonoids on human enterovirus A71 replication. ANTIVIRAL RESEARCH 150 : 60-68. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2017.12.003
Abstract: © 2017 Elsevier B.V. Hand Foot Mouth Disease (HFMD), resulting from human enterovirus A71 (HEVA71) infection can cause severe neurological complications leading to fatality in young children. Currently, there is no approved antiviral for therapeutic treatment against HEVA71 infection. In this study, a 500-compound flavonoid library was screened to identify potential inhibitors of HEVA71 using high-throughput immunofluorescence-based phenotypic screening method. Two lead flavonoid compounds, ST077124 and ST024734 at the non-cytotoxic concentration of 50 μM were found to be effective antivirals that inhibited replication of HEVA71, reducing infectious viral titers by 3.5 log10 PFU/ml and 2.5 log10 PFU/ml respectively. Our study revealed that ST077124 is a specific antiviral compound that inhibits human enteroviruses while ST024734 exhibited antiviral activity against human enteroviruses as well as dengue virus type-2. We also identified that both compounds affected the viral RNA transcription and translation machinery of HEVA71 but did not interfere with the viral internal ribosomal entry site (IRES) activity. Hence, our findings strongly suggest that ST077124 and ST024734 are effective antiviral compounds of minimal cytotoxicity and could serve as promising therapeutic agents against HEVA71 infection.
Source Title: ANTIVIRAL RESEARCH
URI: https://scholarbank.nus.edu.sg/handle/10635/173260
ISSN: 01663542
18729096
DOI: 10.1016/j.antiviral.2017.12.003
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
12.pdfAccepted version4.51 MBAdobe PDF

OPEN

Post-printView/Download

SCOPUSTM   
Citations

24
checked on Aug 7, 2022

Page view(s)

236
checked on Aug 4, 2022

Download(s)

3
checked on Aug 4, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.