Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.chom.2015.06.006
Title: | Co-option of membrane wounding enables virus penetration into cells | Authors: | Luisoni, S Suomalainen, M Boucke, K Tanner, LB Wenk, MR Guan, XL Grzybek, M Coskun, U Greber, UF |
Keywords: | Adenoviridae Biotransformation Capsid Proteins Cell Membrane Ceramides Endocytosis Exocytosis HeLa Cells Host-Pathogen Interactions Humans Lysosomes Sphingomyelin Phosphodiesterase Sphingomyelins Virus Internalization |
Issue Date: | 8-Jul-2015 | Publisher: | Elsevier BV | Citation: | Luisoni, S, Suomalainen, M, Boucke, K, Tanner, LB, Wenk, MR, Guan, XL, Grzybek, M, Coskun, U, Greber, UF (2015-07-08). Co-option of membrane wounding enables virus penetration into cells. Cell Host and Microbe 18 (1) : 75-85. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chom.2015.06.006 | Abstract: | © 2015 Elsevier Inc. During cell entry, non-enveloped viruses undergo partial uncoating to expose membrane lytic proteins for gaining access to the cytoplasm. We report that adenovirus uses membrane piercing to induce and hijack cellular wound removal processes that facilitate further membrane disruption and infection. Incoming adenovirus stimulates calcium influx and lysosomal exocytosis, a membrane repair mechanism resulting in release of acid sphingomyelinase (ASMase) and degradation of sphingomyelin to ceramide lipids in the plasma membrane. Lysosomal exocytosis is triggered by small plasma membrane lesions induced by the viral membrane lytic protein- VI, which is exposed upon mechanical cues from virus receptors, followed by virus endocytosis into leaky endosomes. Chemical inhibition or RNA interference of ASMase slows virus endocytosis, inhibits virus escape to the cytosol, and reduces infection. Ceramide enhances binding of protein-VI to lipid membranes and protein-VI-induced membrane rupture. Thus, adenovirus uses a positive feedback loop between virus uncoating and lipid signaling for efficient membrane penetration. | Source Title: | Cell Host and Microbe | URI: | https://scholarbank.nus.edu.sg/handle/10635/173254 | ISSN: | 19313128 19346069 |
DOI: | 10.1016/j.chom.2015.06.006 |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
4. Co-option of Membrane Wounding Enables Virus Penetration into Cells.pdf | 3.51 MB | Adobe PDF | CLOSED | None |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.