Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbalip.2017.12.010
Title: Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.)
Authors: Woodfield, Helen K
Cazenave-Gassiot, Amaury 
Haslam, Richard P
Guschina, Irina A
Wenk, Markus R 
Harwood, John L
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biophysics
Cell Biology
Developing oilseed rape
Brassica napes (L)
Lipidomics
Lipid accumulation
Regulation of synthesis
METABOLIC-CONTROL ANALYSIS
PLANT OIL COMPOSITION
FATTY-ACID SYNTHESIS
TRIACYLGLYCEROL ACCUMULATION
SHOTGUN LIPIDOMICS
BIOSYNTHESIS
PHOSPHATIDYLCHOLINE
CELLS
PHOSPHOLIPIDS
PATHWAYS
Issue Date: 1-Mar-2018
Publisher: ELSEVIER SCIENCE BV
Citation: Woodfield, Helen K, Cazenave-Gassiot, Amaury, Haslam, Richard P, Guschina, Irina A, Wenk, Markus R, Harwood, John L (2018-03-01). Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.). BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1863 (3) : 339-348. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbalip.2017.12.010
Abstract: © 2018 The Authors With dwindling available agricultural land, concurrent with increased demand for oil, there is much current interest in raising oil crop productivity. We have been addressing this issue by studying the regulation of oil accumulation in oilseed rape (Brassica napus L). As part of this research we have carried out a detailed lipidomic analysis of developing seeds. The molecular species distribution in individual lipid classes revealed quite distinct patterns and showed where metabolic connections were important. As the seeds developed, the molecular species distributions changed, especially in the period of early (20 days after flowering, DAF) to mid phase (27DAF) of oil accumulation. The patterns of molecular species of diacylglycerol, phosphatidylcholine and acyl-CoAs were used to predict the possible relative contributions of diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase to triacylglycerol production. Our calculations suggest that DGAT may hold a more important role in influencing the molecular composition of TAG. Enzyme selectivity had an important influence on the final molecular species patterns. Our data contribute significantly to our understanding of lipid accumulation in the world's third most important oil crop.
Source Title: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
URI: https://scholarbank.nus.edu.sg/handle/10635/170834
ISSN: 13881981
00063002
DOI: 10.1016/j.bbalip.2017.12.010
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