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https://doi.org/10.1016/j.bbalip.2017.12.010
Title: | Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.) | Authors: | Woodfield, Helen K Cazenave-Gassiot, Amaury Haslam, Richard P Guschina, Irina A Wenk, Markus R Harwood, John L |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology Biophysics Cell Biology Developing oilseed rape Brassica napes (L) Lipidomics Lipid accumulation Regulation of synthesis METABOLIC-CONTROL ANALYSIS PLANT OIL COMPOSITION FATTY-ACID SYNTHESIS TRIACYLGLYCEROL ACCUMULATION SHOTGUN LIPIDOMICS BIOSYNTHESIS PHOSPHATIDYLCHOLINE CELLS PHOSPHOLIPIDS PATHWAYS |
Issue Date: | 1-Mar-2018 | Publisher: | ELSEVIER SCIENCE BV | Citation: | Woodfield, Helen K, Cazenave-Gassiot, Amaury, Haslam, Richard P, Guschina, Irina A, Wenk, Markus R, Harwood, John L (2018-03-01). Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.). BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1863 (3) : 339-348. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbalip.2017.12.010 | Abstract: | © 2018 The Authors With dwindling available agricultural land, concurrent with increased demand for oil, there is much current interest in raising oil crop productivity. We have been addressing this issue by studying the regulation of oil accumulation in oilseed rape (Brassica napus L). As part of this research we have carried out a detailed lipidomic analysis of developing seeds. The molecular species distribution in individual lipid classes revealed quite distinct patterns and showed where metabolic connections were important. As the seeds developed, the molecular species distributions changed, especially in the period of early (20 days after flowering, DAF) to mid phase (27DAF) of oil accumulation. The patterns of molecular species of diacylglycerol, phosphatidylcholine and acyl-CoAs were used to predict the possible relative contributions of diacylglycerol acyltransferase (DGAT) and phospholipid:diacylglycerol acyltransferase to triacylglycerol production. Our calculations suggest that DGAT may hold a more important role in influencing the molecular composition of TAG. Enzyme selectivity had an important influence on the final molecular species patterns. Our data contribute significantly to our understanding of lipid accumulation in the world's third most important oil crop. | Source Title: | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | URI: | https://scholarbank.nus.edu.sg/handle/10635/170834 | ISSN: | 13881981 00063002 |
DOI: | 10.1016/j.bbalip.2017.12.010 |
Appears in Collections: | Staff Publications Elements |
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