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https://doi.org/10.1530/JOE-19-0267
Title: | Myo-inositol alters C-13-labeled fatty acid metabolism in human placental explants | Authors: | Watkins, Oliver C Islam, Mohammed Omedul Selvam, Preben Pillai, Reshma Appukuttan Cazenave-Gassiot, Amaury Bendt, Anne K Karnani, Neerja Godfrey, Keith M Lewis, Rohan M Wenk, Markus R Chan, Shiao-Yng |
Keywords: | Science & Technology Life Sciences & Biomedicine Endocrinology & Metabolism placenta pregnancy fatty acids phospholipids metabolism GESTATIONAL DIABETES-MELLITUS ACETYL-COA CARBOXYLASE GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE INSULIN-RESISTANCE OBESITY GROWTH SUPPLEMENTATION ACCUMULATION INHIBITION DEFICIENCY |
Issue Date: | 1-Oct-2019 | Publisher: | BIOSCIENTIFICA LTD | Citation: | Watkins, Oliver C, Islam, Mohammed Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K, Karnani, Neerja, Godfrey, Keith M, Lewis, Rohan M, Wenk, Markus R, Chan, Shiao-Yng (2019-10-01). Myo-inositol alters C-13-labeled fatty acid metabolism in human placental explants. JOURNAL OF ENDOCRINOLOGY 243 (1) : 73-84. ScholarBank@NUS Repository. https://doi.org/10.1530/JOE-19-0267 | Abstract: | We postulate that myo-inositol, a proposed intervention for gestational-diabetes, affects transplacental lipid supply to the fetus. We investigated the effect of myo-inositol on fatty-acid processing in human placental-explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic-acid, 13C-oleic-acid and 13C-docosahexaenoic-acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled-fatty-acids into individual lipids was quantified by liquid-chromatography-mass-spectrometry. At 24 h, myo-inositol increased the amount of 13C-palmitic-acid and 13C-oleic-acid labeled lipids (median fold-change relative to control=1). Significant effects were seen with 30 µM myo-inositol (physiological) for 13C-palmitic-acid-lysophosphatidylcholines (1.26) and 13C-palmitic-acid-phosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased 13C-oleic-acid-lipids but decreased 13C-palmitic-acid and 13C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for 13C-oleic-acid-phosphatidylcholines (1.25), 13C-oleic-acid-phosphatidylethanolamines (1.37) and 13C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for 13C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same 13C-fatty-acid showed similar responses when tested at the same time-point, suggesting myo-inositol alters upstream processes such as fatty-acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences. | Source Title: | JOURNAL OF ENDOCRINOLOGY | URI: | https://scholarbank.nus.edu.sg/handle/10635/170833 | ISSN: | 00220795 14796805 |
DOI: | 10.1530/JOE-19-0267 |
Appears in Collections: | Staff Publications Elements |
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