Please use this identifier to cite or link to this item: https://doi.org/10.1530/JOE-19-0267
Title: Myo-inositol alters C-13-labeled fatty acid metabolism in human placental explants
Authors: Watkins, Oliver C 
Islam, Mohammed Omedul
Selvam, Preben 
Pillai, Reshma Appukuttan 
Cazenave-Gassiot, Amaury 
Bendt, Anne K 
Karnani, Neerja 
Godfrey, Keith M
Lewis, Rohan M
Wenk, Markus R 
Chan, Shiao-Yng 
Keywords: Science & Technology
Life Sciences & Biomedicine
Endocrinology & Metabolism
placenta
pregnancy
fatty acids
phospholipids
metabolism
GESTATIONAL DIABETES-MELLITUS
ACETYL-COA CARBOXYLASE
GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE
INSULIN-RESISTANCE
OBESITY
GROWTH
SUPPLEMENTATION
ACCUMULATION
INHIBITION
DEFICIENCY
Issue Date: 1-Oct-2019
Publisher: BIOSCIENTIFICA LTD
Citation: Watkins, Oliver C, Islam, Mohammed Omedul, Selvam, Preben, Pillai, Reshma Appukuttan, Cazenave-Gassiot, Amaury, Bendt, Anne K, Karnani, Neerja, Godfrey, Keith M, Lewis, Rohan M, Wenk, Markus R, Chan, Shiao-Yng (2019-10-01). Myo-inositol alters C-13-labeled fatty acid metabolism in human placental explants. JOURNAL OF ENDOCRINOLOGY 243 (1) : 73-84. ScholarBank@NUS Repository. https://doi.org/10.1530/JOE-19-0267
Abstract: We postulate that myo-inositol, a proposed intervention for gestational-diabetes, affects transplacental lipid supply to the fetus. We investigated the effect of myo-inositol on fatty-acid processing in human placental-explants from uncomplicated pregnancies. Explants were incubated with 13C-labeled palmitic-acid, 13C-oleic-acid and 13C-docosahexaenoic-acid across a range of myo-inositol concentrations for 24 h and 48 h. The incorporation of labeled-fatty-acids into individual lipids was quantified by liquid-chromatography-mass-spectrometry. At 24 h, myo-inositol increased the amount of 13C-palmitic-acid and 13C-oleic-acid labeled lipids (median fold-change relative to control=1). Significant effects were seen with 30 µM myo-inositol (physiological) for 13C-palmitic-acid-lysophosphatidylcholines (1.26) and 13C-palmitic-acid-phosphatidylethanolamines (1.17). At 48 h, myo-inositol addition increased 13C-oleic-acid-lipids but decreased 13C-palmitic-acid and 13C-docosahexaenoic-acid lipids. Significant effects were seen with 30 µM myo-inositol for 13C-oleic-acid-phosphatidylcholines (1.25), 13C-oleic-acid-phosphatidylethanolamines (1.37) and 13C-oleic-acid-triacylglycerols (1.32) and with 100 µM myo-inositol for 13C-docosahexaenoic-acid-triacylglycerols (0.78). Lipids labeled with the same 13C-fatty-acid showed similar responses when tested at the same time-point, suggesting myo-inositol alters upstream processes such as fatty-acid uptake or activation. Myo-inositol supplementation may alter placental lipid physiology with unknown clinical consequences.
Source Title: JOURNAL OF ENDOCRINOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/170833
ISSN: 00220795
14796805
DOI: 10.1530/JOE-19-0267
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