Please use this identifier to cite or link to this item:
https://doi.org/10.3390/md12010115
Title: | Anti-Chikungunya viral activities of aplysiatoxin-related compounds from the marine cyanobacterium Trichodesmium erythraeum | Authors: | Gupta, DK PARVEEN KAUR Leong, ST Tan, LT Prinsep, MR CHU JANG HANN |
Keywords: | Animals Antiviral Agents Cell Line Cell Line, Tumor Cell Survival Chikungunya virus Cricetinae Cyanobacteria Dose-Response Relationship, Drug Eutrophication Humans Lyngbya Toxins Magnetic Resonance Spectroscopy Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S Viral Plaque Assay |
Issue Date: | 1-Jan-2014 | Publisher: | MDPI AG | Citation: | Gupta, DK, PARVEEN KAUR, Leong, ST, Tan, LT, Prinsep, MR, CHU JANG HANN (2014-01-01). Anti-Chikungunya viral activities of aplysiatoxin-related compounds from the marine cyanobacterium Trichodesmium erythraeum. Marine Drugs 12 (1) : 115-127. ScholarBank@NUS Repository. https://doi.org/10.3390/md12010115 | Abstract: | Tropical filamentous marine cyanobacteria have emerged as a viable source of novel bioactive natural products for drug discovery and development. In the present study, aplysiatoxin (1), debromoaplysiatoxin (2) and anhydrodebromoaplysiatoxin (3), as well as two new analogues, 3-methoxyaplysiatoxin (4) and 3-methoxydebromoaplysiatoxin (5), are reported for the first time from the marine cyanobacterium Trichodesmium erythraeum. The identification of the bloom-forming cyanobacterial strain was confirmed based on phylogenetic analysis of its 16S rRNA sequences. Structural determination of the new analogues was achieved by extensive NMR spectroscopic analysis and comparison with NMR spectral data of known compounds. In addition, the antiviral activities of these marine toxins were assessed using Chikungunya virus (CHIKV)-infected cells. Post-treatment experiments using the debrominated analogues, namely compounds 2, 3 and 5, displayed dose-dependent inhibition of CHIKV when tested at concentrations ranging from 0.1 μM to 10.0 μM. Furthermore, debromoaplysiatoxin (2) and 3-methoxydebromoaplysiatoxin (5) exhibited significant anti-CHIKV activities with EC50 values of 1.3 μM and 2.7 μM, respectively, and selectivity indices of 10.9 and 9.2, respectively. © 2014 by the authors; licensee MDPI. | Source Title: | Marine Drugs | URI: | https://scholarbank.nus.edu.sg/handle/10635/170737 | ISSN: | 1660-3397 | DOI: | 10.3390/md12010115 |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
marinedrugs-12-00115.pdf | Published version | 738.01 kB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.