Please use this identifier to cite or link to this item: https://doi.org/10.1039/c6sc00055j
Title: A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection
Authors: YUAN YOUYONG 
ZHANG RUOYU 
Cheng, Xiamin
XU SHIDANG 
LIU BIN 
Keywords: Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
AGGREGATION-INDUCED-EMISSION
LIGHT-UP PROBE
CANCER-CELLS
IN-SITU
BIOMEDICAL APPLICATIONS
THERAPEUTIC RESPONSES
PHOTODYNAMIC THERAPY
IMAGING PROBES
NANOPARTICLES
APOPTOSIS
Issue Date: 1-Jan-2016
Publisher: Royal Society of Chemistry (RSC)
Citation: YUAN YOUYONG, ZHANG RUOYU, Cheng, Xiamin, XU SHIDANG, LIU BIN (2016-01-01). A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection. Chemical Science 7 (7) : 4245-4250. ScholarBank@NUS Repository. https://doi.org/10.1039/c6sc00055j
Abstract: The accurate detection of biological substances is highly desirable to study various biological processes and evaluate disease progression. Herein, we report a self-validated fluorescent probe which is composed of a coumarin fluorophore as the energy donor and a fluorogen with aggregation-induced emission characteristics (AIEgen) as the energy quencher linked through a caspase-3 specific peptide substrate. Unlike the traditionally widely studied fluorescence resonance energy transfer (FRET) probes, our new generation of FRET probe is non-fluorescent itself due to the energy transfer as well as the dissipation of the acceptor energy through the free molecular motion of AIEgen. Upon interaction with caspase-3, the probe displays strong green and red fluorescent signals synchronously due to the separation of the donor-quencher and aggregation of the released AIEgen. The fluorescence turn-on with dual signal amplification allows real-time and self-validated enzyme detection with a high signal-to-background ratio, providing a good opportunity to accurately monitor various biological processes in a real-time manner.
Source Title: Chemical Science
URI: https://scholarbank.nus.edu.sg/handle/10635/169652
ISSN: 2041-6520
2041-6539
DOI: 10.1039/c6sc00055j
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