Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2019.119615
Title: Bio-orthogonal click reaction-enabled highly specific in situ cellularization of tissue engineering scaffolds
Authors: MAO DUO 
Zhang, Chuangnian
KENRY 
Liu, Jing
Wang, Xiaoxiao
Li, Binhan
Yan, Hongyu
Hu, Fang
Kong, Deling
Wang, Zhihong
LIU BIN 
Keywords: Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
Bio-orthogonal reaction
Tissue engineering
Cellularization
Metabolic glycoengineering
Electrospinning
EXTRACELLULAR-MATRIX
SURFACE MODIFICATION
BIOMATERIALS
FIBERS
Issue Date: 1-Feb-2020
Publisher: Elsevier Ltd
Citation: MAO DUO, Zhang, Chuangnian, KENRY, Liu, Jing, Wang, Xiaoxiao, Li, Binhan, Yan, Hongyu, Hu, Fang, Kong, Deling, Wang, Zhihong, LIU BIN (2020-02-01). Bio-orthogonal click reaction-enabled highly specific in situ cellularization of tissue engineering scaffolds. Biomaterials 230. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2019.119615
Abstract: Tissue engineering generally utilizes natural or synthetic scaffolds to repair or replace damaged tissues. However, due to the lack of guidance of biological signals, most of the implanted scaffolds have always suffered from poor in vivo cellularization. Herein, we demonstrate a bio-orthogonal reaction-based strategy to realize in situ specific and fast cellularization of tissue engineering scaffold. DBCO-modified PCL-PEG (PCL-PEG-DBCO) polymer was synthesized and then fabricated into PCL-PEG-DBCO film through electrospinning. Meanwhile, azide-labeled macrophages (N3 (+) macrophages) were obtained through metabolic glycoengineering. Through a series of in vitro dynamic and in vivo characterization, DBCO-modified films were noted to dramatically increase the selective capture efficiency and survival rate of N3 (+) cells. Additionally, there is negligible influence of covalent conjugation on cell viability and proliferation, indicating the feasibility of the bio-orthogonal click reaction-based tissue engineering strategy. Overall, this work shows the advantages of an in situ bio-orthogonal click reaction in realizing highly specific, efficient, and long-lasting scaffold cellularization. We anticipate that this general strategy would be widely applicable and useful in tissue engineering and regenerative medicine in the near future.
Source Title: Biomaterials
URI: https://scholarbank.nus.edu.sg/handle/10635/169649
ISSN: 0142-9612
1878-5905
DOI: 10.1016/j.biomaterials.2019.119615
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