Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/168891
Title: The major cockroach allergen Bla g 4 binds tyramine and octopamine
Authors: Offermann, Lesa R
Chan, Siew Leong 
Osinski, Tomasz
Tan, Yih Wan
Chew, Fook Tim 
Sivaraman, J 
Mok, Yu-Keung 
Minor, Wladek
Chruszcz, Maksymilian
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Immunology
Cockroach allergen
Bla g 4
Per a 4
Tyramine
Octopamine
Allergy
INNER-CITY CHILDREN
PROTEIN DATA-BANK
BLATTELLA-GERMANICA
POSSIBLE NEUROTRANSMITTER/NEUROMODULATOR
MAXIMUM-LIKELIHOOD
RISK-FACTORS
MODEL
ASTHMA
FAMILIES
EXPOSURE
Issue Date: 1-Jul-2014
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: Offermann, Lesa R, Chan, Siew Leong, Osinski, Tomasz, Tan, Yih Wan, Chew, Fook Tim, Sivaraman, J, Mok, Yu-Keung, Minor, Wladek, Chruszcz, Maksymilian (2014-07-01). The major cockroach allergen Bla g 4 binds tyramine and octopamine. MOLECULAR IMMUNOLOGY 60 (1) : 86-94. ScholarBank@NUS Repository.
Abstract: Bla g 4 is a male cockroach specific protein and is one of the major allergens produced by Blattella germanica (German cockroach). This protein belongs to the lipocalin family that comprises a set of proteins that characteristically bind small hydrophobic molecules and play a role in a number of processes such as: retinoid and pheromone transport, prostaglandin synthesis and mammalian immune response. Using NMR and isothermal titration calorimetry we demonstrated that Bla g 4 binds tyramine and octopamine in solution. In addition, crystal structure analysis of the complex revealed details of tyramine binding. As tyramine and octopamine play important roles in invertebrates, and are counterparts to vertebrate adrenergic transmitters, we speculate that these molecules are physiological ligands for Bla g 4. The nature of binding these ligands to Bla g 4 sheds light on the possible biological function of the protein. In addition, we performed a large-scale analysis of Bla g 4 and Per a 4 (an allergen from American cockroach) homologs to get insights into the function of these proteins. This analysis together with a structural comparison of Blag 4 and Per a 4 suggests that these proteins may play different roles and most likely bind different ligands.Accession numbers: The atomic coordinates and the structure factors have been deposited to the Protein Data Band under accession codes: 4N7C for native Bla g 4 and 4N7D for the Se-Met Bla g 4 structure. © 2014 Elsevier Ltd.
Source Title: MOLECULAR IMMUNOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/168891
ISSN: 01615890
18729142
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