Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gky521
Title: The role of spacer sequence in modulating turn-on fluorescence of DNA-templated silver nanoclusters
Authors: Ang, YS 
Woon, WWE
Yung, LYL 
Keywords: Base Sequence
Cytosine
DNA
Fluorescence
Nanostructures
Nucleotides
Silver
Templates, Genetic
Issue Date: 1-Jan-2018
Publisher: Oxford University Press (OUP)
Citation: Ang, YS, Woon, WWE, Yung, LYL (2018-01-01). The role of spacer sequence in modulating turn-on fluorescence of DNA-templated silver nanoclusters. Nucleic Acids Research 46 (14) : 6974-6982. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gky521
Abstract: © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. Guanine activation of fluorescence in DNA templated silver nanoclusters (AgNCs) is an interesting physical phenomenon which has yet to be fully understood to date. While the individual role of cytosine and guanine has been established, there is still a knowledge gap on how the AgNC-DNA system switches from dark to bright state. Here, we present evidence on the universal role of the DNA spacer sequence in physically separating two Ag+-binding cytosine sites to maintain the dark state while holding them together for structural re-organization by the guanine-rich strand to activate the bright state. The extent of turn-on signal could be modulated by adjusting the spacer length and composition. The ATATA spacer sequence was found to have negligible dark state fluorescence and a turn-on effect of 2440-fold, which was almost five times of the highest factor reported to date.
Source Title: Nucleic Acids Research
URI: https://scholarbank.nus.edu.sg/handle/10635/168694
ISSN: 03051048
13624962
DOI: 10.1093/nar/gky521
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