Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/168646
Title: Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae
Authors: Tan, Kang Wei 
Kumar, Sundramurthy 
Chew, Fook Tim 
Mok, Yu Keung 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Biochemistry & Molecular Biology
Biophysics
Crystallography
group 7 allergens
Der f 7
Dermatophagoides farinae
MONOCLONAL-ANTIBODY BINDING
MOLECULAR-CLONING
CRYSTALS
PROTEINS
EXTRACTS
DER-F-7
ACID
IGE
Issue Date: 1-Dec-2011
Publisher: INT UNION CRYSTALLOGRAPHY
Citation: Tan, Kang Wei, Kumar, Sundramurthy, Chew, Fook Tim, Mok, Yu Keung (2011-12-01). Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 67 (12) : 1612-1615. ScholarBank@NUS Repository.
Abstract: Der f 7 is a major group 7 allergen from the dust mite Dermatophagoides farinae that shows 86% sequence identity to the homologous allergen Der p 7 from D. pteronyssinus. Der f 7 was successfully overexpressed in an Escherichia coli expression system and purified to homogeneity using Ni-NTA affinity and size-exclusion column chromatography. SeMet-labelled Der f 7 was crystallized by the hanging-drop vapour-diffusion method using a reservoir solution consisting of 0.1 M bis-tris pH 7.4 and 28% polyethylene glycol monomethyl ether 2000 at 293 K. X-ray diffraction data were collected to 2.24 Å resolution using synchrotron radiation. The crystals belonged to the orthorhombic system, space group P2 12 12 1, with unit-cell parameters a = 50.19, b = 58.67, c = 123.81 Å. Based on the estimated Matthews coefficient (2.16 Å 3 Da -1), two molecules of Der f 7 could be present in the asymmetric unit of the crystal lattice. © 2011 International Union of Crystallography. All rights reserved.
Source Title: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
URI: https://scholarbank.nus.edu.sg/handle/10635/168646
ISSN: 17443091
2053230X
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