Please use this identifier to cite or link to this item:
|Title:||Hevin plays a pivotal role in corneal wound healing||Authors:||Chaurasia S.S.
|Keywords:||collagen type 1
collagen type 4
ophthalmic excimer laser
Extracellular Matrix Proteins
In Situ Nick-End Labeling
|Issue Date:||2013||Publisher:||Public Library of Science||Citation:||Chaurasia S.S., Perera P.R., Poh R., Lim R.R., Wong T.T., Mehta J.S. (2013). Hevin plays a pivotal role in corneal wound healing. PLoS ONE 8 (11) : e81544. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0081544||Abstract:||Background: Hevin is a matricellular protein involved in tissue repair and remodeling via interaction with the surrounding extracellular matrix (ECM) proteins. In this study, we examined the functional role of hevin using a corneal stromal wound healing model achieved by an excimer laser-induced irregular phototherapeutic keratectomy (IrrPTK) in hevin-null (hevin -/-) mice. We also investigated the effects of exogenous supplementation of recombinant human hevin (rhHevin) to rescue the stromal cellular components damaged by the excimer laser. Methodology/Principal Findings: Wild type (WT) and hevin-/- mice were divided into three groups at 4 time points-1, 2, 3 and 4 weeks. Group I served as naïve without any treatment. Group II received epithelial debridement and underwent IrrPTK using excimer laser. Group III received topical application of rhHevin after IrrPTK surgery for 3 days. Eyes were analyzed for corneal haze and matrix remodeling components using slit lamp biomicroscopy, in vivo confocal microscopy, light microscopy (LM), transmission electron microscopy (TEM), immunohistochemistry (IHC) and western blotting (WB). IHC showed upregulation of hevin in IrrPTK-injured WT mice. Hevin-/- mice developed corneal haze as early as 1-2 weeks post IrrPTK-treatment compared to the WT group, which peaked at 3-4 weeks. They also exhibited accumulation of inflammatory cells, fibrotic components of ECM proteins and vascularized corneas as seen by IHC and WB. LM and TEM showed activated keratocytes (myofibroblasts), inflammatory debris and vascular tissues in the stroma. Exogenous application of rhHevin for 3 days reinstated inflammatory index of the corneal stroma similar to WT mice. Conclusions/Significance: Hevin is transiently expressed in the IrrPTK-injured corneas and loss of hevin predisposes them to aberrant wound healing. Hevin -/- mice develop early corneal haze characterized by severe chronic inflammation and stromal fibrosis that can be rescued with exogenous administration of rhHevin. Thus, hevin plays a pivotal role in the corneal wound healing. Copyright: © 2013 Chaurasia et al.||Source Title:||PLoS ONE||URI:||https://scholarbank.nus.edu.sg/handle/10635/165965||ISSN:||19326203||DOI:||10.1371/journal.pone.0081544|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
|10_1371_journal_pone_0081544.pdf||3.05 MB||Adobe PDF|
checked on Apr 7, 2020
checked on Apr 2, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.