Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0182984
Title: The effect of Bacopa monnieri on gene expression levels in SH-SY5Y human neuroblastoma cells
Authors: Leung H.-W. 
Foo G. 
Banumurthy G. 
Chai X. 
Ghosh S. 
Mitra-Ganguli T.
Vandongen A.M.J. 
Keywords: Bacopa monnieri extract
transcriptome
messenger RNA
Article
Bacopa monnieri
controlled study
gene expression regulation
gene identification
human
human cell
membrane transport
neuroprotection
oxidative stress
protein misfolding
quantitative analysis
reverse transcription polymerase chain reaction
RNA sequence
SH-SY5Y cell line
transcriptomics
translation regulation
Bacopa
cell differentiation
genetics
metabolism
neuroblastoma
pathology
physiology
sequence analysis
tumor cell line
Bacopa
Cell Differentiation
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Humans
Neuroblastoma
RNA, Messenger
Sequence Analysis, RNA
Issue Date: 2017
Publisher: Public Library of Science
Citation: Leung H.-W., Foo G., Banumurthy G., Chai X., Ghosh S., Mitra-Ganguli T., Vandongen A.M.J. (2017). The effect of Bacopa monnieri on gene expression levels in SH-SY5Y human neuroblastoma cells. PLoS ONE 12 (8) : e0182984. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0182984
Abstract: Bacopa monnieri is a plant used as a nootropic in Ayurveda, a 5000-year-old system of traditional Indian medicine. Although both animal and clinical studies supported its role as a memory enhancer, the molecular and cellular mechanism underlying Bacopa’s nootropic action are not understood. In this study, we used deep sequencing (RNA-Seq) to identify the transcriptome changes upon Bacopa treatment on SH-SY5Y human neuroblastoma cells. We identified several genes whose expression levels were regulated by Bacopa. Biostatistical analysis of the RNA-Seq data identified biological pathways and molecular functions that were regulated by Bacopa, including regulation of mRNA translation and transmembrane transport, responses to oxidative stress and protein misfolding. Pathway analysis using the Ingenuity platform suggested that Bacopa may protect against brain damage and improve brain development. These newly identified molecular and cellular determinants may contribute to the nootropic action of Bacopa and open up a new direction of investigation into its mechanism of action. © 2017 Leung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165781
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0182984
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