Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pntd.0003445
Title: Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients
Authors: Kam Y.-W.
Pok K.-Y.
Eng K.E.
Tan L.-K.
Kaur S.
Lee W.W.L.
Leo Y.-S. 
Ng L.-C. 
Ng L.F.P. 
Keywords: anti E2EP3 antibody
antibody
immunoglobulin G
immunoglobulin M
unclassified drug
biological marker
immunoglobulin G
viral protein
virus antibody
animal cell
antibody detection
antibody specificity
Arbovirus
Article
Barmah Forest virus
Chikungunya alphavirus
clinical article
controlled study
cross reaction
Dengue virus
enzyme linked immunosorbent assay
Flavivirus
immunofluorescence test
immunoreactivity
longitudinal study
nonhuman
protein analysis
reverse transcription polymerase chain reaction
Ross River alpha virus
seroprevalence
virus infection
virus neutralization
blood
Chikungunya virus
epidemiology
human
immunology
virology
virus infection
Antibodies, Viral
Antibody Specificity
Arbovirus Infections
Biomarkers
Chikungunya virus
Cross Reactions
Humans
Immunoglobulin G
Seroepidemiologic Studies
Viral Proteins
Issue Date: 2015
Publisher: Public Library of Science
Citation: Kam Y.-W., Pok K.-Y., Eng K.E., Tan L.-K., Kaur S., Lee W.W.L., Leo Y.-S., Ng L.-C., Ng L.F.P. (2015). Sero-Prevalence and Cross-Reactivity of Chikungunya Virus Specific Anti-E2EP3 Antibodies in Arbovirus-Infected Patients. PLoS Neglected Tropical Diseases 9 (1) : 10. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pntd.0003445
Abstract: Chikungunya virus (CHIKV) and clinically-related arboviruses cause large epidemics with serious economic and social impact. As clinical symptoms of CHIKV infections are similar to several flavivirus infections, good detection methods to identify CHIKV infection are desired for improved treatment and clinical management. The strength of anti-E2EP3 antibody responses was explored in a longitudinal study on 38 CHIKV-infected patients. We compared their anti-E2EP3 responses with those of patients infected with non-CHIKV alphaviruses, or flaviviruses. E2EP3 cross-reactive samples from patients infected with non-CHIKV viruses were further analyzed with an in vitro CHIKV neutralization assay. CHIKV-specific anti-E2EP3 antibody responses were detected in 72% to 100% of patients. Serum samples from patients infected with other non-CHIKV alphaviruses were cross-reactive to E2EP3. Interestingly, some of these antibodies demonstrated clearly in vitro CHIKV neutralizing activity. Contrastingly, serum samples from flaviviruses-infected patients showed a low level of cross-reactivity against E2EP3. Using CHIKV E2EP3 as a serology marker not only allows early detection of CHIKV specific antibodies, but would also allow the differentiation between CHIKV infections and flavivirus infections with 93% accuracy, thereby allowing precise acute febrile diagnosis and improving clinical management in regions newly suffering from CHIKV outbreaks including the Americas. © 2015 Kam et al.
Source Title: PLoS Neglected Tropical Diseases
URI: https://scholarbank.nus.edu.sg/handle/10635/165395
ISSN: 19352727
DOI: 10.1371/journal.pntd.0003445
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