Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.ppat.1004972
Title: Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development
Authors: Deng Y.Z.
Qu Z.
Naqvi N.I. 
Keywords: transcription factor SNF
fungal protein
acetylation
Article
circadian rhythm
controlled study
darkness
fungal development
fungal gene
fungal metabolism
genetic regulation
habitual adaptation
homeostasis
Magnaporthe oryzae
nonhuman
phosphorylation
phototropism
Twilight gene
circadian rhythm
drug effects
food
fungus hyphae
fungus spore
gene expression regulation
genetics
growth, development and aging
homeostasis
light
Magnaporthe
metabolism
microbiology
Oryza
oxidation reduction reaction
phototropism
physiology
plant disease
Fungi
Magnaporthe grisea
Magnaporthe oryzae
Circadian Rhythm
Food
Fungal Proteins
Gene Expression Regulation, Fungal
Homeostasis
Hyphae
Light
Magnaporthe
Oryza
Oxidation-Reduction
Phototropism
Plant Diseases
Spores, Fungal
Issue Date: 2015
Publisher: Public Library of Science
Citation: Deng Y.Z., Qu Z., Naqvi N.I. (2015). Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development. PLoS Pathogens 11 (6) : e1004972. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.ppat.1004972
Abstract: Phototropic regulation of circadian clock is important for environmental adaptation, organismal growth and differentiation. Light plays a critical role in fungal development and virulence. However, it is unclear what governs the intracellular metabolic response to such dark-light rhythms in fungi. Here, we describe a novel circadian-regulated Twilight (TWL) function essential for phototropic induction of asexual development and pathogenesis in the rice-blast fungus Magnaporthe oryzae. The TWL transcript oscillates during circadian cycles and peaks at subjective twilight. GFP-Twl remains acetylated and cytosolic in the dark, whereas light-induced phosphorylation (by the carbon sensor Snf1 kinase) drives it into the nucleus. The mRNA level of the transcription/repair factor TFB5, was significantly down regulated in the twl∆ mutant. Overexpression of TFB5 significantly suppressed the conidiation defects in the twl∆ mutant. Furthermore, Tfb5-GFP translocates to the nucleus during the phototropic response and under redox stress, while it failed to do so in the twl∆ mutant. Thus, we provide mechanistic insight into Twl-based regulation of nutrient and redox homeostasis in response to light during pathogen adaptation to the host milieu in the rice blast pathosystem. © 2015 Deng et al.
Source Title: PLoS Pathogens
URI: https://scholarbank.nus.edu.sg/handle/10635/165390
ISSN: 15537366
DOI: 10.1371/journal.ppat.1004972
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