Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2202-13-64
Title: Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain
Authors: Parakalan, Rangarajan 
Jiang, Boran 
Nimmi, Baby 
Janani, Manivannan 
Jayapal, Manikandan
Lu, Jia 
Tay, Samuel SW 
Ling, Eng-Ang 
Dheen, S Thameem 
Keywords: Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
MICROTUBULE-ASSOCIATED PROTEIN
LYMPHOCYTE-SPECIFIC PROTEIN-1
CELL-DIFFERENTIATION
REACTIVE MICROGLIA
TRANSGENIC MICE
NERVOUS-SYSTEM
GOLLI PROTEINS
WHITE-MATTER
KINASE-II
EXPRESSION
Issue Date: 14-Jun-2012
Publisher: BMC
Citation: Parakalan, Rangarajan, Jiang, Boran, Nimmi, Baby, Janani, Manivannan, Jayapal, Manikandan, Lu, Jia, Tay, Samuel SW, Ling, Eng-Ang, Dheen, S Thameem (2012-06-14). Transcriptome analysis of amoeboid and ramified microglia isolated from the corpus callosum of rat brain. BMC NEUROSCIENCE 13 (1). ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2202-13-64
Abstract: Background: Microglia, the resident immune cells of the central nervous system (CNS), have two distinct phenotypes in the developing brain: amoeboid form, known to be amoeboid microglial cells (AMC) and ramified form, known to be ramified microglial cells (RMC). The AMC are characterized by being proliferative, phagocytic and migratory whereas the RMC are quiescent and exhibit a slow turnover rate. The AMC transform into RMC with advancing age, and this transformation is indicative of the gradual shift in the microglial functions. Both AMC and RMC respond to CNS inflammation, and they become hypertrophic when activated by trauma, infection or neurodegenerative stimuli. The molecular mechanisms and functional significance of morphological transformation of microglia during normal development and in disease conditions is not clear. It is hypothesized that AMC and RMC are functionally regulated by a specific set of genes encoding various signaling molecules and transcription factors.Results: To address this, we carried out cDNA microarray analysis using lectin-labeled AMC and RMC isolated from frozen tissue sections of the corpus callosum of 5-day and 4-week old rat brain respectively, by laser capture microdissection. The global gene expression profiles of both microglial phenotypes were compared and the differentially expressed genes in AMC and RMC were clustered based on their functional annotations. This genome wide comparative analysis identified genes that are specific to AMC and RMC.Conclusions: The novel and specific molecules identified from the trancriptome explains the quiescent state functioning of microglia in its two distinct morphological states. © 2012 Rangarajan et al.; licensee BioMed Central Ltd.
Source Title: BMC NEUROSCIENCE
URI: https://scholarbank.nus.edu.sg/handle/10635/163899
ISSN: 14712202
14712202
DOI: 10.1186/1471-2202-13-64
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