Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/163898
Title: Zinc supplementation prevents cardiomyocyte apoptosis and congenital heart defects in embryos of diabetic mice
Authors: Kumar, Srinivasan Dinesh 
Vijaya, Murugaiyan 
Samy, Ramar Perumal 
Dheen, S Thameem 
Ren, Minqin 
Watt, Frank 
Kang, Y James
Bay, Boon-Huat 
Tay, Samuel Sam Wah 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Endocrinology & Metabolism
Cardiac malformations
Maternal diabetes
Oxidative stress
Antioxidant
Zinc
Metallothionein
Nuclear microscopy
Heart
Embryos
Mice
RADICAL SCAVENGING ENZYMES
ENDOTHELIAL GROWTH-FACTOR
OXIDATIVE STRESS
LIPID-PEROXIDATION
NITRIC-OXIDE
DOXORUBICIN CARDIOTOXICITY
PREGNANT-WOMEN
CORD PLASMA
CELL-DEATH
METALLOTHIONEIN
Issue Date: 15-Oct-2012
Publisher: ELSEVIER SCIENCE INC
Citation: Kumar, Srinivasan Dinesh, Vijaya, Murugaiyan, Samy, Ramar Perumal, Dheen, S Thameem, Ren, Minqin, Watt, Frank, Kang, Y James, Bay, Boon-Huat, Tay, Samuel Sam Wah (2012-10-15). Zinc supplementation prevents cardiomyocyte apoptosis and congenital heart defects in embryos of diabetic mice. Free Radical Biology and Medicine 53 (8) : 1595-1606. ScholarBank@NUS Repository.
Abstract: Oxidative stress induced by maternal diabetes plays an important role in the development of cardiac malformations. Zinc (Zn) supplementation of animals and humans has been shown to ameliorate oxidative stress induced by diabetic cardiomyopathy. However, the role of Zn in the prevention of oxidative stress induced by diabetic cardiac embryopathy remains unknown. We analyzed the preventive role of Zn in diabetic cardiac embryopathy by both in vivo and in vitro studies. In vivo study revealed a significant decrease in lipid peroxidation, superoxide ions, and oxidized glutathione and an increase in reduced glutathione, nitric oxide, and superoxide dismutase in the developing heart at embryonic days (E) 13.5 and 15.5 in the Zn-supplemented diabetic group when compared to the diabetic group. In addition, significantly down-regulated protein and mRNA expression of metallothionein (MT) in the developing heart of embryos from diabetic group was rescued by Zn supplement. Further, the nuclear microscopy results showed that trace elements such as phosphorus, calcium, and Zn levels were significantly increased (P<0.001), whereas the iron level was significantly decreased (P<0.05) in the developing heart of embryos from the Zn-supplemented diabetic group. In vitro study showed a significant increase in cellular apoptosis and the generation of reactive oxygen species (ROS) in H9c2 (rat embryonic cardiomyoblast) cells exposed to high glucose concentrations. Supplementation with Zn significantly decreased apoptosis and reduced the levels of ROS. In summary, oxidative stress induced by maternal diabetes could play a role in the development and progression of cardiac embryopathy, and Zn supplementation could be a potential therapy for diabetic cardiac embryopathy. © 2012 Elsevier Inc.
Source Title: Free Radical Biology and Medicine
URI: https://scholarbank.nus.edu.sg/handle/10635/163898
ISSN: 08915849
18734596
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