Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.gene.2012.12.071
Title: Differential gene expression profiles during embryonic heart development in diabetic mice pregnancy
Authors: Vijaya, Murugaiyan 
Manikandan, Jayapal 
Parakalan, Rangarajan 
Dheen, S Thameem 
Kumar, Srinivasan Dinesh 
Tay, Samuel Sam Wah 
Keywords: Science & Technology
Life Sciences & Biomedicine
Genetics & Heredity
Microarray
Diabetes
Heart
Embryo
Mice
CONGENITAL-MALFORMATIONS
N-CADHERIN
CARDIOVASCULAR MALFORMATIONS
BIRTH-DEFECTS
OUTFLOW TRACT
INFANTS
GROWTH
SPECIFICATION
MODULATION
ANOMALIES
Issue Date: 10-Mar-2013
Publisher: ELSEVIER
Citation: Vijaya, Murugaiyan, Manikandan, Jayapal, Parakalan, Rangarajan, Dheen, S Thameem, Kumar, Srinivasan Dinesh, Tay, Samuel Sam Wah (2013-03-10). Differential gene expression profiles during embryonic heart development in diabetic mice pregnancy. GENE 516 (2) : 218-227. ScholarBank@NUS Repository. https://doi.org/10.1016/j.gene.2012.12.071
Abstract: Congenital heart defects (CHD) are one of the most common defects in offspring of diabetic mothers. There is a clear association between maternal diabetes and CHD; however the underlying molecular mechanism remains unknown. We hypothesized that maternal diabetes affects with the expression of early developmental genes that regulate the essential developmental processes of the heart, thereby resulting in the pathogenesis of CHD. We analyzed genome-wide expression profiling in the developing heart of embryos from diabetic and control mice by using the oligonucleotide microarray. Microarray analysis revealed that a total of 878 genes exhibited more than 1.5 fold changes in expression level in the hearts of experimental embryos in either E13.5 or E15.5 compared with their respective controls. Expression pattern of genes that is differentially expressed in the developing heart was further examined by the real-time reverse transcriptase-polymerase chain reaction. Several genes involved in a number of molecular signaling pathways such as apoptosis, proliferation, migration and differentiation in the developing heart were differentially expressed in embryos of diabetic pregnancy. It is concluded that altered expression of several genes involved in heart development may contribute to CHD in offspring of diabetic mothers. © 2012 Elsevier B.V.
Source Title: GENE
URI: https://scholarbank.nus.edu.sg/handle/10635/163896
ISSN: 03781119
18790038
DOI: 10.1016/j.gene.2012.12.071
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