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https://doi.org/10.1016/j.gene.2012.12.071
Title: | Differential gene expression profiles during embryonic heart development in diabetic mice pregnancy | Authors: | Vijaya, Murugaiyan Manikandan, Jayapal Parakalan, Rangarajan Dheen, S Thameem Kumar, Srinivasan Dinesh Tay, Samuel Sam Wah |
Keywords: | Science & Technology Life Sciences & Biomedicine Genetics & Heredity Microarray Diabetes Heart Embryo Mice CONGENITAL-MALFORMATIONS N-CADHERIN CARDIOVASCULAR MALFORMATIONS BIRTH-DEFECTS OUTFLOW TRACT INFANTS GROWTH SPECIFICATION MODULATION ANOMALIES |
Issue Date: | 10-Mar-2013 | Publisher: | ELSEVIER | Citation: | Vijaya, Murugaiyan, Manikandan, Jayapal, Parakalan, Rangarajan, Dheen, S Thameem, Kumar, Srinivasan Dinesh, Tay, Samuel Sam Wah (2013-03-10). Differential gene expression profiles during embryonic heart development in diabetic mice pregnancy. GENE 516 (2) : 218-227. ScholarBank@NUS Repository. https://doi.org/10.1016/j.gene.2012.12.071 | Abstract: | Congenital heart defects (CHD) are one of the most common defects in offspring of diabetic mothers. There is a clear association between maternal diabetes and CHD; however the underlying molecular mechanism remains unknown. We hypothesized that maternal diabetes affects with the expression of early developmental genes that regulate the essential developmental processes of the heart, thereby resulting in the pathogenesis of CHD. We analyzed genome-wide expression profiling in the developing heart of embryos from diabetic and control mice by using the oligonucleotide microarray. Microarray analysis revealed that a total of 878 genes exhibited more than 1.5 fold changes in expression level in the hearts of experimental embryos in either E13.5 or E15.5 compared with their respective controls. Expression pattern of genes that is differentially expressed in the developing heart was further examined by the real-time reverse transcriptase-polymerase chain reaction. Several genes involved in a number of molecular signaling pathways such as apoptosis, proliferation, migration and differentiation in the developing heart were differentially expressed in embryos of diabetic pregnancy. It is concluded that altered expression of several genes involved in heart development may contribute to CHD in offspring of diabetic mothers. © 2012 Elsevier B.V. | Source Title: | GENE | URI: | https://scholarbank.nus.edu.sg/handle/10635/163896 | ISSN: | 03781119 18790038 |
DOI: | 10.1016/j.gene.2012.12.071 |
Appears in Collections: | Staff Publications Elements |
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