Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/162753
Title: CHARACTERISATION OF A HUMAN ANTIBODY RESPONSE THAT LINKS CYTOMEGALOVIRUS INFECTION WITH SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: NEO JIE YING JACKLYN
Keywords: Human cytomegalovirus, Antibody, Systemic lupus erythematosus
Issue Date: 10-Jun-2019
Citation: NEO JIE YING JACKLYN (2019-06-10). CHARACTERISATION OF A HUMAN ANTIBODY RESPONSE THAT LINKS CYTOMEGALOVIRUS INFECTION WITH SYSTEMIC LUPUS ERYTHEMATOSUS. ScholarBank@NUS Repository.
Abstract: Human cytomegalovirus (HCMV) is a ubiquitous herpesvirus that has been linked with systemic lupus erythematosus (SLE) pathogenesis. This study focuses on the detailed characterisation of 3A11, an antibody specific for UL44. UL44 encodes the HCMV DNA polymerase processivity factor, a nuclear-resident, non-structural protein vital for HCMV DNA replication. The intracellular nature of this viral protein complicates its targeting by the humoral response. 3A11 immunoprecipitates UL44 from HCMV-infected cells with known nuclear SLE autoantigens – namely, nucleolin, dsDNA and ku70. We show that UL44 is redistributed to the cell surface during virus-induced apoptosis with these autoantigens. In a cohort of SLE patients, we also show significant associations between anti-HCMV IgG and anti-UL44 IgG antibody levels with SLE. Taken together, these results suggest a new mechanism where host nuclear antigens may be adjuvantised through their co-expression on the cell surface with a HCMV viral antigen, potentially increasing the risk of SLE development.
URI: https://scholarbank.nus.edu.sg/handle/10635/162753
Appears in Collections:Ph.D Theses (Open)

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