Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/162753
DC FieldValue
dc.titleCHARACTERISATION OF A HUMAN ANTIBODY RESPONSE THAT LINKS CYTOMEGALOVIRUS INFECTION WITH SYSTEMIC LUPUS ERYTHEMATOSUS
dc.contributor.authorNEO JIE YING JACKLYN
dc.date.accessioned2019-12-12T18:02:38Z
dc.date.available2019-12-12T18:02:38Z
dc.date.issued2019-06-10
dc.identifier.citationNEO JIE YING JACKLYN (2019-06-10). CHARACTERISATION OF A HUMAN ANTIBODY RESPONSE THAT LINKS CYTOMEGALOVIRUS INFECTION WITH SYSTEMIC LUPUS ERYTHEMATOSUS. ScholarBank@NUS Repository.
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/162753
dc.description.abstractHuman cytomegalovirus (HCMV) is a ubiquitous herpesvirus that has been linked with systemic lupus erythematosus (SLE) pathogenesis. This study focuses on the detailed characterisation of 3A11, an antibody specific for UL44. UL44 encodes the HCMV DNA polymerase processivity factor, a nuclear-resident, non-structural protein vital for HCMV DNA replication. The intracellular nature of this viral protein complicates its targeting by the humoral response. 3A11 immunoprecipitates UL44 from HCMV-infected cells with known nuclear SLE autoantigens – namely, nucleolin, dsDNA and ku70. We show that UL44 is redistributed to the cell surface during virus-induced apoptosis with these autoantigens. In a cohort of SLE patients, we also show significant associations between anti-HCMV IgG and anti-UL44 IgG antibody levels with SLE. Taken together, these results suggest a new mechanism where host nuclear antigens may be adjuvantised through their co-expression on the cell surface with a HCMV viral antigen, potentially increasing the risk of SLE development.
dc.language.isoen
dc.subjectHuman cytomegalovirus, Antibody, Systemic lupus erythematosus
dc.typeThesis
dc.contributor.departmentMICROBIOLOGY & IMMUNOLOGY
dc.contributor.supervisorPAUL A MACARY
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
NeoJYJ.pdf5.3 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

15
checked on Feb 27, 2020

Download(s)

7
checked on Feb 27, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.