Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0048737
Title: Sequence of closely related plasmids encoding bla(NDM-1) in two unrelated Klebsiella pneumoniae isolates in Singapore.
Authors: Chen Y.T.
Lin A.C.
Siu L.K.
Koh T.H. 
Keywords: antiinfective agent
beta lactamase
beta lactamase NDM 1
beta-lactamase NDM-1
adult
article
bacterium conjugation
case report
drug effect
enzymology
genetics
human
isolation and purification
Klebsiella pneumoniae
male
microbial sensitivity test
molecular genetics
nucleotide sequence
plasmid
Singapore
Adult
Anti-Bacterial Agents
Base Sequence
beta-Lactamases
Conjugation, Genetic
Humans
Klebsiella pneumoniae
Male
Microbial Sensitivity Tests
Molecular Sequence Annotation
Molecular Sequence Data
Plasmids
Singapore
Young Adult
Issue Date: 2012
Citation: Chen Y.T., Lin A.C., Siu L.K., Koh T.H. (2012). Sequence of closely related plasmids encoding bla(NDM-1) in two unrelated Klebsiella pneumoniae isolates in Singapore.. PloS one 7 (11). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0048737
Rights: Attribution 4.0 International
Abstract: Spread of the bla(NDM-1) gene that encodes the New Delhi metallo-?-lactamase (NDM-1) in Enterobacteriaceae is a major global health problem. Plasmids carrying bla(NDM-1) from two different multi-drug resistant Klebsiella pneumonia isolates collected in Singapore were completely sequenced and compared to known plasmids carrying bla(NDM-1). The two plasmids, pTR3 and pTR4, were transferred to Escherichia coli recipient strain J53 and completely sequenced by a shotgun approach using 3-kb paired-end libraries on 454. Although the K. pneumoniae strains were unrelated by molecular typing using PFGE and MLST, complete sequencing revealed that pTR3 and pTR4 are identical. The plasmid sequence is similar to the E. coli NDM-1-encoding plasmid p271A, which was isolated in Australia from a patient returning from Bangladesh. The immediate regions of the bla(NDM-1) gene in pTR3/4 are identical to that of p271A, but the backbone of our plasmid is much more similar to another IncN2 plasmid reported recently, pJIE137, which contained an additional 5.2-kb CUP (conserved upstream repeat) regulon region in comparison to p271A. A 257-bp element bounded by imperfect 39-bp inverted repeats (IR) and an incomplete version of this element flanking the 3.6-kb NDM-1-encoding region were identified in these plasmids and are likely to be the vestiges of an unknown IS. Although the hosts are not epidemiologically linked, we found that the plasmids bearing the bla(NDM-1) gene are identical. Comparative analyses of the conserved NDM-1-encoding region among different plasmids from K. pneumoniae and E. coli suggested that the transposable elements and the two unknown IR-associated elements flanking the NDM-1-encoding region might have aided the spreading of this worrisome resistance determinant.
Source Title: PloS one
URI: https://scholarbank.nus.edu.sg/handle/10635/162014
ISSN: 19326203
DOI: 10.1371/journal.pone.0048737
Rights: Attribution 4.0 International
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