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https://doi.org/10.1371/journal.pone.0029250
Title: | Seasonal variation in vitamin D 3 levels is paralleled by changes in the peripheral blood human T cell compartment | Authors: | Khoo A.-L. Koenen H.J.P.M. Chai L.Y.A. Sweep F.C.G.J. Netea M.G. van der Ven A.J.A.M. Joosten I. |
Keywords: | calcifediol calcitriol chemokine receptor CCR4 chemokine receptor CCR6 chemokine receptor CCR7 chemokine receptor CCR9 colecalciferol cutaneous lymphocyte associated antigen transcription factor FOXP3 colecalciferol cytokine adult article CD4+ T lymphocyte CD8+ T lymphocyte cell homing controlled study cytokine production flow cytometry human human cell lymphocyte count lymphocyte migration male normal human peripheral blood mononuclear cell peripheral lymphocyte protein expression regulatory T lymphocyte seasonal variation T lymphocyte vitamin blood level autoimmune disease biosynthesis blood cytology environmental exposure middle aged mononuclear cell radiation exposure season sunlight T lymphocyte subpopulation Adult Autoimmune Diseases Cholecalciferol Cytokines Environmental Exposure Humans Leukocytes, Mononuclear Male Middle Aged Seasons Sunlight T-Lymphocyte Subsets |
Issue Date: | 2012 | Citation: | Khoo A.-L., Koenen H.J.P.M., Chai L.Y.A., Sweep F.C.G.J., Netea M.G., van der Ven A.J.A.M., Joosten I. (2012). Seasonal variation in vitamin D 3 levels is paralleled by changes in the peripheral blood human T cell compartment. PLoS ONE 7 (1) : e29250. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0029250 | Rights: | Attribution 4.0 International | Abstract: | It is well-recognized that vitamin D 3 has immune-modulatory properties and that the variation in ultraviolet (UV) exposure affects vitamin D 3 status. Here, we investigated if and to what extent seasonality of vitamin D 3 levels are associated with changes in T cell numbers and phenotypes. Every three months during the course of the entire year, human PBMC and whole blood from 15 healthy subjects were sampled and analyzed using flow cytometry. We observed that elevated serum 25(OH)D 3 and 1,25(OH) 2 D 3 levels in summer were associated with a higher number of peripheral CD4 + and CD8 + T cells. In addition, an increase in naïve CD4 + CD45RA + T cells with a reciprocal drop in memory CD4 + CD45RO + T cells was observed. The increase in CD4 + CD45RA + T cell count was a result of heightened proliferative capacity rather than recent thymic emigration of T cells. The percentage of Treg dropped in summer, but not the absolute Treg numbers. Notably, in the Treg population, the levels of forkhead box protein 3 (Foxp3) expression were increased in summer. Skin, gut and lymphoid tissue homing potential was increased during summer as well, exemplified by increased CCR4, CCR6, CLA, CCR9 and CCR7 levels. Also, in summer, CD4 + and CD8 + T cells revealed a reduced capacity to produce pro-inflammatory cytokines. In conclusion, seasonal variation in vitamin D 3 status in vivo throughout the year is associated with changes in the human peripheral T cell compartment and may as such explain some of the seasonal variation in immune status which has been observed previously. Given that the current observations are limited to healthy adult males, larger population-based studies would be useful to validate these findings. © 2012 Khoo et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/162006 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0029250 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
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