Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0034589
Title: Inhibition of enterovirus 71 (EV-71) infections by a novel antiviral peptide derived from EV-71 capsid protein VP1
Authors: Tan C.W.
Chan Y.F.
Sim K.M.
Tan E.L. 
Poh C.L.
Keywords: antivirus agent
arginine
lysine
protein VP1
SP40 peptide
SP40X peptide
unclassified drug
alanine
capsid protein
oligopeptide
amino acid substitution
animal cell
antiviral activity
article
cancer cell
concentration response
controlled study
Coxsackie virus A
drug mechanism
drug specificity
Enterovirus 71
Enterovirus infection
female
genotype
human
human cell
IC 50
in vitro study
nonhuman
nucleotide sequence
Poliomyelitis virus 1
rhabdomyosarcoma
structure activity relation
virus attachment
amino acid sequence
animal
cell proliferation
cell strain HT29
cell survival
Cercopithecus
chemical structure
chemistry
drug effect
Enterovirus
Enterovirus infection
genetics
growth, development and aging
HeLa cell
molecular genetics
peptide library
protein conformation
protein secondary structure
sequence homology
synthesis
tumor cell line
Vero cell
virology
Human enterovirus 71
Human poliovirus 1
Miridae
Alanine
Amino Acid Sequence
Amino Acid Substitution
Animals
Antiviral Agents
Capsid Proteins
Cell Line, Tumor
Cell Proliferation
Cell Survival
Cercopithecus aethiops
Enterovirus A, Human
Enterovirus Infections
HeLa Cells
HT29 Cells
Humans
Inhibitory Concentration 50
Models, Molecular
Molecular Sequence Data
Oligopeptides
Peptide Library
Protein Conformation
Protein Structure, Secondary
Sequence Homology, Amino Acid
Vero Cells
Issue Date: 2012
Citation: Tan C.W., Chan Y.F., Sim K.M., Tan E.L., Poh C.L. (2012). Inhibition of enterovirus 71 (EV-71) infections by a novel antiviral peptide derived from EV-71 capsid protein VP1. PLoS ONE 7 (5) : e34589. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0034589
Abstract: Enterovirus 71 (EV-71) is the main causative agent of hand, foot and mouth disease (HFMD). In recent years, EV-71 infections were reported to cause high fatalities and severe neurological complications in Asia. Currently, no effective antiviral or vaccine is available to treat or prevent EV-71 infection. In this study, we have discovered a synthetic peptide which could be developed as a potential antiviral for inhibition of EV-71. Ninety five synthetic peptides (15-mers) overlapping the entire EV-71 capsid protein, VP1, were chemically synthesized and tested for antiviral properties against EV-71 in human Rhabdomyosarcoma (RD) cells. One peptide, SP40, was found to significantly reduce cytopathic effects of all representative EV-71 strains from genotypes A, B and C tested, with IC50 values ranging from 6-9.3 ?M in RD cells. The in vitro inhibitory effect of SP40 exhibited a dose dependent concentration corresponding to a decrease in infectious viral particles, total viral RNA and the levels of VP1 protein. The antiviral activity of SP40 peptide was not restricted to a specific cell line as inhibition of EV-71 was observed in RD, HeLa, HT-29 and Vero cells. Besides inhibition of EV-71, it also had antiviral activities against CV-A16 and poliovirus type 1 in cell culture. Mechanism of action studies suggested that the SP40 peptide was not virucidal but was able to block viral attachment to the RD cells. Substitutions of arginine and lysine residues with alanine in the SP40 peptide at positions R3A, R4A, K5A and R13A were found to significantly decrease antiviral activities, implying the importance of positively charged amino acids for the antiviral activities. The data demonstrated the potential and feasibility of SP40 as a broad spectrum antiviral agent against EV-71. © 2012 Tan et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161985
ISSN: 19326203
DOI: 10.1371/journal.pone.0034589
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