Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.1006698
Title: VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane
Authors: Gordon D.E.
Chia J.
Jayawardena K.
Antrobus R.
Bard F. 
Peden A.A.
Keywords: cellubrevin
SNAP24 protein
SNAP29 protein
SNARE protein
synaptobrevin
syntaxin 1
syntaxin 4
unclassified drug
YKT6 protein
cellubrevin
Drosophila protein
Snap24 protein, Drosophila
SNARE protein
soluble n ethylmaleimide sensitive factor attachment protein
synaptobrevin
synaptobrevin protein, Drosophila
verotoxin 1
animal cell
Article
cell membrane
controlled study
genetic conservation
Golgi complex
membrane fusion
membrane transport
nonhuman
protein depletion
RNA interference
secretory vesicle
animal
cell membrane
Drosophila melanogaster
genetics
heterozygote
human
membrane fusion
metabolism
protein transport
Animals
Cell Membrane
Drosophila melanogaster
Drosophila Proteins
Golgi Apparatus
Heterozygote
Humans
Membrane Fusion
Protein Transport
R-SNARE Proteins
RNA Interference
Shiga Toxin 1
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Vesicle-Associated Membrane Protein 3
Issue Date: 2017
Citation: Gordon D.E., Chia J., Jayawardena K., Antrobus R., Bard F., Peden A.A. (2017). VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane. PLoS Genetics 13 (4) : e1006698. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.1006698
Rights: Attribution 4.0 International
Abstract: The cellular machinery required for the fusion of constitutive secretory vesicles with the plasma membrane in metazoans remains poorly defined. To address this problem we have developed a powerful, quantitative assay for measuring secretion and used it in combination with combinatorial gene depletion studies in Drosophila cells. This has allowed us to identify at least three SNARE complexes mediating Golgi to PM transport (STX1, SNAP24/29 and Syb; STX1, SNAP24/29 and YKT6; STX4, SNAP24 and Syb). RNAi mediated depletion of YKT6 and VAMP3 in mammalian cells also blocks constitutive secretion suggesting that YKT6 has an evolutionarily conserved role in this process. The unexpected role of YKT6 in plasma membrane fusion may in part explain why RNAi and gene disruption studies have failed to produce the expected phenotypes in higher eukaryotes. ? 2017 Gordon et al.
Source Title: PLoS Genetics
URI: https://scholarbank.nus.edu.sg/handle/10635/161895
ISSN: 15537390
DOI: 10.1371/journal.pgen.1006698
Rights: Attribution 4.0 International
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