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https://doi.org/10.1371/journal.pone.0016637
Title: | Running worms: C. elegans self-sorting by electrotaxis | Authors: | Manière X. Lebois F. Matic I. Ladoux B. Di Meglio J.-M. Hersen P. |
Keywords: | aging analytic method article Caenorhabditis elegans comparative study controlled study electric field electrophoresis electrotaxis fitness gene mutation high throughput screening image analysis locomotion methodology nonhuman phenotype quantitative analysis simulation velocity acceleration animal animal behavior biological model electrostimulation escape behavior evaluation genetics high throughput screening locomotion motor performance physiology running transgenic animal validation study Caenorhabditis elegans Nematoda Acceleration Aging Animals Animals, Genetically Modified Behavior, Animal Caenorhabditis elegans Electric Stimulation Escape Reaction High-Throughput Screening Assays Locomotion Models, Biological Motor Skills Running |
Issue Date: | 2011 | Citation: | Manière X., Lebois F., Matic I., Ladoux B., Di Meglio J.-M., Hersen P. (2011). Running worms: C. elegans self-sorting by electrotaxis. PLoS ONE 6 (2) : e16637. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0016637 | Rights: | Attribution 4.0 International | Abstract: | The nematode C. elegans displays complex dynamical behaviors that are commonly used to identify relevant phenotypes. Although its maintenance is straightforward, sorting large populations of worms when looking for a behavioral phenotype is difficult, time consuming and hardly quantitative when done manually. Interestingly, when submitted to a moderate electric field, worms move steadily along straight trajectories. Here, we report an inexpensive method to measure worms crawling velocities and sort them within a few minutes by taking advantage of their electrotactic skills. This method allows to quantitatively measure the effect of mutations and aging on worm's crawling velocity. We also show that worms with different locomotory phenotypes can be spatially sorted, fast worms traveling away from slow ones. Group of nematodes with comparable locomotory fitness could then be isolated for further analysis. C. elegans is a growing model for neurodegenerative diseases and using electrotaxis for self-sorting can improve the high-throughput search of therapeutic bio-molecules. © 2011 Manière et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161787 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0016637 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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