Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0116047
Title: Production of novel antibiotics zeamines through optimizing Dickeya zeae fermentation conditions
Authors: Liao L.
Cheng Y.
Liu S.
Zhou J.
An S.
Lv M.
Chen Y.
Gu Y.
Chen S.
Zhang L.-H. 
Keywords: antibiotic agent
aspartic acid
magnesium ion
nitrate
phosphate
potassium ion
sucrose
unclassified drug
zeamine
zeamine II
antiinfective agent
macrolide
polyamine
zeamine
antibiotic biosynthesis
Article
Bacteria
bacterial gene
bacterial strain
bacterial virulence
carbon source
controlled study
culture medium
Dickeya zeae
fermentation medium
fermentation optimization
gene expression
host pathogen interaction
minimum inhibitory concentration
nonhuman
regulatory mechanism
response surface method
zmsK gene
culture technique
Enterobacteriaceae
fermentation
metabolism
microbiology
Bacteria (microorganisms)
Dickeya zeae
Negibacteria
Posibacteria
Anti-Bacterial Agents
Cell Culture Techniques
Enterobacteriaceae
Fermentation
Industrial Microbiology
Macrolides
Polyamines
Issue Date: 2014
Citation: Liao L., Cheng Y., Liu S., Zhou J., An S., Lv M., Chen Y., Gu Y., Chen S., Zhang L.-H. (2014). Production of novel antibiotics zeamines through optimizing Dickeya zeae fermentation conditions. PLoS ONE 9 (12) : e116047. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0116047
Abstract: Dickeya zeae strain EC1 was recently shown to produce a new type of phytotoxins designated as zeamine and zeamine II, which are potent wide-spectrum antibiotics against Gram-positive and Gram-negative bacterial pathogens, suggesting their promising potential as clinical medicines. In this study, the optimized medium composition and culture conditions for biosynthesis of novel antibiotics zeamines have been established by using response surface methodology, largely increasing the yield of zeamines from original about 7.35 µg·mL−1 in minimal medium to about 150 µg·mL−1 in LS5 medium. The study identified the major factors contributing to zeamines production, which include nitrate, sucrose, asparaginate, mineral elements Mg2+ and K+, and optimized amount of phosphate. In addition, the results showed that overexpression of zmsK in D. zeae strain EC1 could further increase zeamines yield to about 180 µg·mL−1 in LS5 medium. The findings from this study could facilitate further characterization and utilization of these two novel antibiotics, and also provide useful clues for understanding the regulatory mechanisms that govern D. zeae virulence. © 2014 Liao et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161753
ISSN: 19326203
DOI: 10.1371/journal.pone.0116047
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0116047.pdf808.06 kBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

14
checked on Aug 5, 2020

Page view(s)

70
checked on Aug 6, 2020

Download(s)

1
checked on Aug 6, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.