Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0051605
Title: From Penicillin-Streptomycin to Amikacin-Vancomycin: Antibiotic Decontamination of Cardiovascular Homografts in Singapore
Authors: Heng W.L.
Lim C.H.
Tan B.H. 
Chlebicki M.P. 
Lee W.H.L.
Seck T.
Lim Y.P.
Keywords: amikacin
aminoglycoside antibiotic agent
amoxicillin plus clavulanic acid
cloxacillin
gentamicin
glycopeptide
penicillin derivative
piperacillin plus tazobactam
streptomycin
vancomycin
Acinetobacter
aerobic cell culture
alpha hemolytic Streptococcus
anaerobic cell culture
Aspergillus fumigatus
Bacillus subtilis
bactericidal activity
bacterium isolate
Candida tropicalis
coagulase negative Staphylococcus
controlled study
diffusion
drug potency
heart transplantation
human
incubation time
Propionibacterium acnes
retrospective study
review
Rhodococcus
Singapore
sporogenesis
Staphylococcus aureus
tissue culture
turbidimetry
Amikacin
Anti-Bacterial Agents
Bacteria
Blood Vessel Prosthesis
Decontamination
Humans
Microbial Sensitivity Tests
Penicillins
Singapore
Streptomycin
Temperature
Time Factors
Transplantation, Homologous
Vancomycin
Bacillus subtilis
Staphylococcus aureus
Issue Date: 2012
Citation: Heng W.L., Lim C.H., Tan B.H., Chlebicki M.P., Lee W.H.L., Seck T., Lim Y.P. (2012). From Penicillin-Streptomycin to Amikacin-Vancomycin: Antibiotic Decontamination of Cardiovascular Homografts in Singapore. PLoS ONE 7 (12) : e51605. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0051605
Abstract: Background: In February 2012, the National Cardiovascular Homograft Bank (NCHB) became the first tissue bank outside of North America to receive accreditation from the American Association of Tissue Banks. From 2008 to 2009, NCHB had been decontaminating its cardiovascular homografts with penicillin and streptomycin. The antibiotic decontamination protocol was changed in January 2010 as amikacin and vancomycin were recommended, in order to cover bacteria isolated from post-recovery and post- antibiotic incubation tissue cultures. Aim: The objective of this study is to determine the optimal incubation conditions for decontamination of homografts by evaluating the potencies of amikacin and vancomycin in different incubation conditions. Retrospective reviews of microbiological results were also performed for homografts recovered from 2008 to 2012, to compare the effectiveness of penicillin-streptomycin versus the amikacin-vancomycin regimens. Methods: Based on microbiological assays stated in United States Pharmacopeia 31, potency of amikacin was evaluated by turbidimetric assay using Staphylococcus aureus, while vancomycin was by diffusion assay using Bacillus subtilis sporulate. Experiments were performed to investigate the potencies of individual antibiotic 6-hours post incubation at 4°C and 37°C and 4°C for 24 hours, after the results suggested that amikacin was more potent at lower temperature. Findings: Tissue incubation at 4°C for 24 hours is optimal for both antibiotics, especially for amikacin, as its potency falls drastically at 37°C. Conclusion: The decontamination regimen of amikacin-vancomycin at 4°C for 24 hours is effective. Nevertheless, it is imperative to monitor microbiological trends closely and evaluate the efficacy of current antibiotics regimen against emerging strains of micro-organisms. © 2012 Heng et al.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161695
ISSN: 19326203
DOI: 10.1371/journal.pone.0051605
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0051605.pdf158.85 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

2
checked on Sep 13, 2020

Page view(s)

87
checked on Sep 18, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.