Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0143539
Title: Respiratory DC use IFITM3 to avoid direct viral infection and safeguard virus-specific CD8+ T cell priming
Authors: Infusini G.
Smith J.M.
Yuan H.
Pizzolla A.
Ng W.C. 
Londrigan S.L.
Haque A.
Reading P.C.
Villadangos J.A.
Wakim L.M.
Keywords: interferon
interferon induced transmembrane protein 3
interferon regulatory factor 3
interferon regulatory factor 7
membrane protein
unclassified drug
virus antigen
fragilis protein, mouse
interferon regulatory factor 3
interferon regulatory factor 7
Irf3 protein, mouse
Irf7 protein, mouse
membrane protein
adaptive immunity
animal cell
Article
CD8+ T lymphocyte
controlled study
dendritic cell
draining lymph node
infection sensitivity
influenza
lymph node
lymphocyte function
lymphocyte migration
mouse
nonhuman
protein expression
protein transport
respiratory dendritic cell
respiratory system
signal transduction
T lymphocyte priming
upregulation
animal
CD8+ T lymphocyte
cell culture
dendritic cell
flow cytometry
genetics
immunology
knockout mouse
metabolism
orthomyxovirus infection
Western blotting
Animals
Blotting, Western
CD8-Positive T-Lymphocytes
Cells, Cultured
Dendritic Cells
Flow Cytometry
Interferon Regulatory Factor-3
Interferon Regulatory Factor-7
Membrane Proteins
Mice
Mice, Knockout
Orthomyxoviridae Infections
Issue Date: 2015
Citation: Infusini G., Smith J.M., Yuan H., Pizzolla A., Ng W.C., Londrigan S.L., Haque A., Reading P.C., Villadangos J.A., Wakim L.M. (2015). Respiratory DC use IFITM3 to avoid direct viral infection and safeguard virus-specific CD8+ T cell priming. PLoS ONE 10 (11) : e0143539. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0143539
Rights: Attribution 4.0 International
Abstract: Respiratory dendritic cells (DC) play a pivotal role in the initiation of adaptive immune responses to influenza virus. To do this, respiratory DCs must ferry viral antigen from the lung to the draining lymph node without becoming infected and perishing en route. We show that respiratory DCs up-regulate the expression of the antiviral molecule, interferoninduced transmembrane protein 3 (IFITM3) in response to influenza virus infection, in a manner dependent on type I interferon signaling and the transcription factors IRF7 and IRF3. Failure of respiratory DCs to up-regulate IFITM3 following influenza virus infection resulted in impaired trafficking to the draining LN and consequently in impaired priming of an influenza-specific CD8+ T cell response. The impaired trafficking of IFITM3-deficient DC correlated with an increased susceptibility of these DC to influenza virus infection. This work shows that the expression of IFITM3 protects respiratory DCs from influenza virus infection, permitting migration from lung to LN and optimal priming of a virus specific T-cell response. � 2015 Infusini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161604
ISSN: 19326203
DOI: 10.1371/journal.pone.0143539
Rights: Attribution 4.0 International
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