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Title: Human mesenchymal stem cells retain multilineage differentiation capacity including neural marker expression after extended in vitro expansion
Authors: Okolicsanyi R.K.
Camilleri E.T.
Oikari L.E.
Yu C.
Cool S.M. 
Van Wijnen A.J.
Griffiths L.R.
Haupt L.M.
Keywords: 5' nucleotidase
beta1 integrin
CD146 antigen
CD200 antigen
CD24 antigen
CD45 antigen
CD56 antigen
Hermes antigen
kruppel like factor 4
Thy 1 antigen
transcription factor HES 1
transcription factor PAX3
transcription factor PAX9
transcription factor Sox5
transcription factor Sox6
transcription factor Sox9
biological marker
CD45 antigen
transcription factor
bone development
cell differentiation
cell expansion
cell growth
cell lineage
controlled study
fluorescence activated cell sorting
gene expression
human cell
in vitro study
mesenchymal stem cell
neural stem cell
polymerase chain reaction
protein expression
quantitative analysis
stem cell culture
young adult
cell culture
cell lineage
cell membrane
cell proliferation
cell shape
flow cytometry
gene expression profiling
gene expression regulation
mesenchymal stroma cell
multicellular spheroid
nerve cell
Western blotting
Antigens, CD45
Blotting, Western
Cell Differentiation
Cell Lineage
Cell Membrane
Cell Proliferation
Cell Shape
Cells, Cultured
Flow Cytometry
Gene Expression Profiling
Gene Expression Regulation
Mesenchymal Stromal Cells
Spheroids, Cellular
Transcription Factors
Issue Date: 2015
Citation: Okolicsanyi R.K., Camilleri E.T., Oikari L.E., Yu C., Cool S.M., Van Wijnen A.J., Griffiths L.R., Haupt L.M. (2015). Human mesenchymal stem cells retain multilineage differentiation capacity including neural marker expression after extended in vitro expansion. PLoS ONE 10 (9) : e0137255. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The suitability of human mesenchymal stem cells (hMSCs) in regenerative medicine relies on retention of their proliferative expansion potential in conjunction with the ability to differentiate toward multiple lineages. Successful utilisation of these cells in clinical applications linked to tissue regeneration requires consideration of biomarker expression, time in culture and donor age, as well as their ability to differentiate towards mesenchymal (bone, cartilage, fat) or non-mesenchymal (e.g., neural) lineages. To identify potential therapeutic suitability we examined hMSCs after extended expansion including morphological changes, potency (stemness) and multilineage potential. Commercially available hMSC populations were expanded in vitro for > 20 passages, equating to > 60 days and > 50 population doublings. Distinct growth phases (A-C) were observed during serial passaging and cells were characterised for stemness and lineage markers at representative stages (Phase A: P+5, approximately 13 days in culture; Phase B: P+7, approximately 20 days in culture; and Phase C: P+13, approximately 43 days in culture). Cell surface markers, stem cell markers and lineage-specific markers were characterised by FACS, ICC and Q-PCR revealing MSCs maintained their multilineage potential, including neural lineages throughout expansion. Co-expression of multiple lineage markers along with continued CD45 expression in MSCs did not affect completion of osteogenic and adipogenic specification or the formation of neuro-spheres. Improved standardised isolation and characterisation of MSCs may facilitate the identification of biomarkers to improve therapeutic efficacy to ensure increased reproducibility and routine production of MSCs for therapeutic applications including neural repair. Copyright: © 2015 Okolicsanyi et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0137255
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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