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Title: Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF
Authors: Ye L. 
Zhang S.
Greder L.
Dutton J.
Keirstead S.A.
Lepley M.
Zhang L.
Kaufman D.
Zhang J.
Keywords: vasculotropin
animal cell
cell differentiation
cell regeneration
cell stimulation
cell structure
controlled study
gene expression
heart muscle cell
heart muscle potential
human cell
nucleotide sequence
pluripotent stem cell
structure analysis
Bone Morphogenetic Protein 4
Cell Differentiation
Cell Line
Cell Lineage
Coculture Techniques
Fetal Blood
Gene Expression Regulation, Developmental
Induced Pluripotent Stem Cells
Leukocytes, Mononuclear
Myocytes, Cardiac
Vascular Endothelial Growth Factor A
Issue Date: 2013
Citation: Ye L., Zhang S., Greder L., Dutton J., Keirstead S.A., Lepley M., Zhang L., Kaufman D., Zhang J. (2013). Effective Cardiac Myocyte Differentiation of Human Induced Pluripotent Stem Cells Requires VEGF. PLoS ONE 8 (1) : e53764. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Perhaps one of the most significant achievements in modern science is the discovery of human induced pluripotent stem cells (hiPSCs), which have paved the way for regeneration therapy using patients' own cells. Cardiomyocytes differentiated from hiPSCs (hiPSC-CMs) could be used for modelling patients with heart failure, for testing new drugs, and for cellular therapy in the future. However, the present cardiomyocyte differentiation protocols exhibit variable differentiation efficiency across different hiPSC lines, which inhibit the application of this technology significantly. Here, we demonstrate a novel myocyte differentiation protocol that can yield a significant, high percentage of cardiac myocyte differentiation (>85%) in 2 hiPSC lines, which makes the fabrication of a human cardiac muscle patch possible. The established hiPSCs cell lines being examined include the transgene integrated UCBiPS7 derived from cord blood cells and non-integrated PCBC16iPS from skin fibroblasts. The results indicate that hiPSC-CMs derived from established hiPSC lines respond to adrenergic or acetylcholine stimulation and beat regularly for greater than 60 days. This data also demonstrates that this novel differentiation protocol can efficiently generate hiPSC-CMs from iPSC lines that are derived not only from fibroblasts, but also from blood mononuclear cells. © 2013 Ye et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0053764
Rights: Attribution 4.0 International
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