Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0064731
Title: | Clonal Expansion within Pneumococcal Serotype 6C after Use of Seven-Valent Vaccine | Authors: | Loman N.J. Gladstone R.A. Constantinidou C. Tocheva A.S. Jefferies J.M.C. Faust S.N. O'Connor L. Chan J. Pallen M.J. Clarke S.C. |
Keywords: | lantibiotic membrane protein Pneumococcus vaccine article bacterial genome bacterial strain bacterial transmission bacterial virulence bacterium carrier bacterium isolation child gene locus gene sequence genetic variability human molecular cloning multilocus sequence typing nonhuman phylogenetic tree preschool child Streptococcus pneumoniae Streptococcus pneumoniae serotype 6C Cell Proliferation Clone Cells Genetic Loci Genome, Bacterial Humans Phylogeny Pneumococcal Infections Pneumococcal Vaccines Polymorphism, Single Nucleotide Sequence Analysis, DNA Serotyping Streptococcus pneumoniae Streptococcus pneumoniae |
Issue Date: | 2013 | Citation: | Loman N.J., Gladstone R.A., Constantinidou C., Tocheva A.S., Jefferies J.M.C., Faust S.N., O'Connor L., Chan J., Pallen M.J., Clarke S.C. (2013). Clonal Expansion within Pneumococcal Serotype 6C after Use of Seven-Valent Vaccine. PLoS ONE 8 (5) : e64731. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0064731 | Rights: | Attribution 4.0 International | Abstract: | Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design. © 2013 Loman et al. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161313 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0064731 | Rights: | Attribution 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0064731.pdf | 350.28 kB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License