Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0179991
Title: Actinic keratosis modelling in mice: A translational study
Authors: Pillon A.
Gomes B.
Vandenberghe I.
Cartron V.
Cèbe P.
Blanchet J.-C.
Sibaud V.
Guilbaud N.
Audoly L. 
Lamant L.
Kruczynski A.
Keywords: CD3 antigen
fluorouracil
ingenol mebutate
Ki 67 antigen
protein p53
fluorouracil
immunosuppressive agent
acanthosis
actinic keratosis
aged
animal cell
animal experiment
animal model
animal tissue
Article
basement membrane
cancer model
clinical article
controlled study
dermatoscope
disease classification
disease course
drug efficacy
histopathology
human
human cell
human tissue
human versus animal comparison
immunohistochemistry
keratinocyte
light exposure
mouse
mouse model
nonhuman
phenotype
protein expression
reproducibility
skin carcinoma
skin defect
translational research
ultraviolet B radiation
very elderly
animal
comparative study
disease model
epiluminescence microscopy
hairless mouse
Keratosis, Actinic
pathology
ultraviolet radiation
Animals
Dermoscopy
Disease Models, Animal
Fluorouracil
Humans
Immunosuppressive Agents
Keratosis, Actinic
Mice
Mice, Hairless
Translational Medical Research
Ultraviolet Rays
Issue Date: 2017
Citation: Pillon A., Gomes B., Vandenberghe I., Cartron V., Cèbe P., Blanchet J.-C., Sibaud V., Guilbaud N., Audoly L., Lamant L., Kruczynski A. (2017). Actinic keratosis modelling in mice: A translational study. PLoS ONE 12 (6) : e0179991. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0179991
Rights: Attribution 4.0 International
Abstract: Background Actinic keratoses (AK) are pre-malignant cutaneous lesions caused by prolonged exposure to ultraviolet radiation. As AKs lesions are generally accepted to be the initial lesions in a disease continuum that progresses to squamous cell carcinoma (SCC), AK lesions have to be treated. They are also the second most common reason for visits to the dermatologist. Several treatments are available but their efficacy still needs to be improved. The UV-B-induced KA lesion mouse model is used in preclinical studies to assess the efficacy of novel molecules, even though it is often more representative of advanced AK or SCC. Objectives Here we report on a translational study, comparing the various stages of AK development in humans and in the UV-B irradiated mouse model, as well as the optimization of photograph acquisition of AK lesions on mouse skin. Methods Human and mouse skin lesions were analysed by histology and immunohistochemistry. Mouse lesions were also assessed using a digital dermatoscope. Results An histological and phenotypic analysis, including p53, Ki67 and CD3 expression detection, performed on human and mouse AK lesions, shows that overall AK modelling in mice is relevant in the clinical situation. Some differences are observed, such as disorganization of keratinocytes of the basal layer and a number of atypical nuclei which are more numerous in human AK, whereas much more pronounced acanthosis is observed in skin lesion in mice. Thanks to this translational study, we are able to select appropriate experimental conditions for establishing either early or advanced stage AK or an SCC model. Furthermore, we optimized photograph acquisition of AK lesions on mouse skin by using a digital dermatoscope which is also used in clinics and allows reproducible photograph acquisition for further reliable assessment of mouse lesions. Use of this camera is illustrated through a pharmacological study assessing the activity of CARAC®. Conclusion These data demonstrate that this mouse model of UV-B-induced skin lesions is predictive for the identification of novel therapeutic treatments for both early and advanced stages of the disease. © 2017 Pillon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/161189
ISSN: 19326203
DOI: 10.1371/journal.pone.0179991
Rights: Attribution 4.0 International
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