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https://doi.org/10.1371/journal.pone.0181155
Title: | A complex association between DNA methylation and gene expression in human placenta at first and third trimesters | Authors: | Lim Y.C. Li J. Ni Y. Liang Q. Zhang J. Yeo G.S.H. Lyu J. Jin S. Ding C. |
Keywords: | DNA messenger RNA transcriptome Article cell cycle controlled study DNA methylation down regulation female first trimester pregnancy gene expression gene ontology gestational age human human tissue immune response placenta promoter region RNA sequence third trimester pregnancy chemistry CpG island first trimester pregnancy gene expression profiling gene expression regulation genetic epigenesis genetics high throughput sequencing placenta pregnancy procedures sequence analysis third trimester pregnancy CpG Islands DNA Methylation Epigenesis, Genetic Female Gene Expression Profiling Gene Expression Regulation, Developmental High-Throughput Nucleotide Sequencing Humans Placenta Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Third Promoter Regions, Genetic Sequence Analysis, RNA |
Issue Date: | 2017 | Citation: | Lim Y.C., Li J., Ni Y., Liang Q., Zhang J., Yeo G.S.H., Lyu J., Jin S., Ding C. (2017). A complex association between DNA methylation and gene expression in human placenta at first and third trimesters. PLoS ONE 12 (7) : e0181155. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0181155 | Rights: | Attribution 4.0 International | Abstract: | The human placenta is a maternal-fetal organ essential for normal fetal development and maternal health. During pregnancy, the placenta undergoes many structural and functional changes in response to fetal needs and environmental exposures. Previous studies have demonstrated widespread epigenetic and gene expression changes from early to late pregnancy. However, on the global level, how DNA methylation changes impact on gene expression in human placenta is not yet well understood. We performed DNA methylome analysis by reduced representation bisulfite sequencing (RRBS) and gene expression analysis by RNA-Seq for both first and third trimester human placenta tissues. From first to third trimester, 199 promoters (corresponding to 189 genes) and 2,297 gene bodies were differentially methylated, with a clear dominance of hypermethylation (96.8% and 93.0% for promoters and gene bodies, respectively). A total of 2,447 genes were differentially expressed, of which 77.2% were down-regulated. Gene ontology analysis using differentially expressed genes were enriched for cell cycle and immune response functions. The correlation between DNA methylation and gene expression was non-linear and complex, depending on the genomic context (promoter or gene body) and gene expression levels. A wide range of DNA methylation and gene expression changes were observed at different gestational ages. The non-linear association between DNA methylation and gene expression indicates that epigenetic regulation of placenta development is more complex than previously envisioned. © 2017 Lim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | Source Title: | PLoS ONE | URI: | https://scholarbank.nus.edu.sg/handle/10635/161182 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0181155 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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