Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scib.2019.02.019
Title: NIR-II fluorescence in vivo confocal microscopy with aggregation-induced emission dots
Authors: YU, W
GUO, B 
ZHANG, H
ZHOU, J
YU, X
ZHU, L
XUE, D
LIU, W
SUN, X
QIAN, J
Issue Date: 30-Mar-2019
Publisher: Elsevier BV
Citation: YU, W, GUO, B, ZHANG, H, ZHOU, J, YU, X, ZHU, L, XUE, D, LIU, W, SUN, X, QIAN, J (2019-03-30). NIR-II fluorescence in vivo confocal microscopy with aggregation-induced emission dots. Science Bulletin 64 (6) : 410-416. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scib.2019.02.019
Abstract: © 2019 Science China Press Significantly reduced tissue scattering of fluorescence signals in the second near-infrared (NIR-II, 1,000–1,700 nm) spectral region offers opportunities for large-depth in vivo bioimaging. Nowadays, most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection (e.g., via InGaAs camera), which has high temporal resolution but limited spatial resolution due to out-of-focus signals. Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues. In this presented work, a NIR-II fluorescence confocal microscopic system was setup. By using a kind of aggregation-induced emission (AIE) dots as NIR-II fluorescent probes, 800 μm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained, and the spatial resolution at 700 μm depth could reach 8.78 μm. Moreover, the time-correlated single photon counting (TCSPC) technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy, and in vivo confocal NIR-II fluorescence lifetime microscopic imaging (FLIM) of mouse cerebral vasculature was successfully realized.
Source Title: Science Bulletin
URI: https://scholarbank.nus.edu.sg/handle/10635/155304
ISSN: 20959273
20959281
DOI: 10.1016/j.scib.2019.02.019
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