Please use this identifier to cite or link to this item: https://doi.org/10.1083/jcb.201503047
Title: Arl2-and Msps-dependent microtubule growth governs asymmetric division
Authors: Chen, Keng 
Koe, Chwee Tat 
Xing, Zhanyuan Benny
Tian, Xiaolin
Rossi, Fabrizio
Wang, Cheng 
Tang, Quan
Zong, Wenhui
Hong, Wan Jin 
Taneja, Reshma 
Yu, Fengwei 
Gonzalez, Cayetano
Wu, Chunlai
Endow, Sharyn 
Wang, Hongyan 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
NEURAL STEM-CELLS
REGULATES SPINDLE ORIENTATION
TUBULIN-FOLDING COFACTORS
FACTOR-LIKE PROTEIN-2
DROSOPHILA NEUROBLASTS
SELF-RENEWAL
NEURONAL MORPHOGENESIS
NETWORK FORMATION
BRAIN-DEVELOPMENT
PROGENITOR CELLS
Issue Date: 14-Mar-2016
Publisher: ROCKEFELLER UNIV PRESS
Citation: Chen, Keng, Koe, Chwee Tat, Xing, Zhanyuan Benny, Tian, Xiaolin, Rossi, Fabrizio, Wang, Cheng, Tang, Quan, Zong, Wenhui, Hong, Wan Jin, Taneja, Reshma, Yu, Fengwei, Gonzalez, Cayetano, Wu, Chunlai, Endow, Sharyn, Wang, Hongyan (2016-03-14). Arl2-and Msps-dependent microtubule growth governs asymmetric division. JOURNAL OF CELL BIOLOGY 212 (6) : 661-676. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.201503047
Abstract: © 2016 Chen et al. Asymmetric division of neural stem cells is a fundamental strategy to balance their self-renewal and differentiation. It is long thought that microtubules are not essential for cell polarity in asymmetrically dividing Drosophila melanogaster neuroblasts (NBs; neural stem cells). Here, we show that Drosophila ADP ribosylation factor like-2 (Arl2) and Msps, a known microtubule-binding protein, control cell polarity and spindle orientation of NBs. Upon arl2 RNA intereference, Arl2-GDP expression, or arl2 deletions, microtubule abnormalities and asymmetric division defects were observed. Conversely, overactivation of Arl2 leads to microtubule overgrowth and depletion of NBs. Arl2 regulates microtubule growth and asymmetric division through localizing Msps to the centrosomes in NBs. Moreover, Arl2 regulates dynein function and in turn centrosomal localization of D-TACC and Msps. Arl2 physically associates with tubulin cofactors C, D, and E. Arl2 functions together with tubulin-binding cofactor D to control microtubule growth, Msps localization, and NB self-renewal. Therefore, Arl2-and Msps-dependent microtubule growth is a new paradigm regulating asymmetric division of neural stem cells.
Source Title: JOURNAL OF CELL BIOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/155227
ISSN: 00219525
15408140
DOI: 10.1083/jcb.201503047
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