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Title: Chromatin immunoprecipitation in skeletal myoblasts
Authors: Rao, VK 
Shankar, SR
Taneja, R 
Keywords: Chromatin modifiers
Histone modifications
Issue Date: 1-Jan-2019
Publisher: Springer New York
Citation: Rao, VK, Shankar, SR, Taneja, R (2019-01-01). Chromatin immunoprecipitation in skeletal myoblasts. Methods in Molecular Biology 1889 : 43-54. ScholarBank@NUS Repository.
Abstract: © Springer Science+Business Media, LLC, part of Springer Nature 2019. Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.
Source Title: Methods in Molecular Biology
ISBN: 9781493988969
DOI: 10.1007/978-1-4939-8897-6_4
Appears in Collections:Staff Publications

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