Please use this identifier to cite or link to this item: https://doi.org/10.1186/s40168-019-0644-3
Title: Interactions between a pathogenic Blastocystis subtype and gut microbiota: in vitro and in vivo studies
Authors: Yason, John Anthony 
Liang, Yi Ran
Png, Chin Wen 
Zhang, Yongliang 
Tan, Kevin Shyong Wei 
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
Blastocystis
Subtypes
Gut microbiota
Dysbiosis
Bifidobacterium
IRRITABLE-BOWEL-SYNDROME
CLINICAL-SIGNIFICANCE
ULCERATIVE-COLITIS
HOMINIS
DYSBIOSIS
BACTERIA
COMMON
MACROPHAGES
METABOLISM
PROBIOTICS
Issue Date: 11-Mar-2019
Publisher: BMC
Citation: Yason, John Anthony, Liang, Yi Ran, Png, Chin Wen, Zhang, Yongliang, Tan, Kevin Shyong Wei (2019-03-11). Interactions between a pathogenic Blastocystis subtype and gut microbiota: in vitro and in vivo studies. MICROBIOME 7 (1). ScholarBank@NUS Repository. https://doi.org/10.1186/s40168-019-0644-3
Abstract: © 2019 The Author(s). Background: Blastocystis is a common gut eukaryote detected in humans and animals. It has been associated with gastrointestinal disease in the past although recent metagenomic studies also suggest that it is a member of normal microbiota. This study investigates interactions between pathogenic human isolates belonging to Blastocystis subtype 7 (ST7) and bacterial representatives of the gut microbiota. Results: Generally, Blastocystis ST7 exerts a positive effect on the viability of representative gut bacteria except on Bifidobacterium longum. Gene expression analysis and flow cytometry indicate that the bacterium may be undergoing oxidative stress in the presence of Blastocystis. In vitro assays demonstrate that Blastocystis-induced host responses are able to decrease Bifidobacterium counts. Mice infected with Blastocystis also reveal a decrease in beneficial bacteria Bifidobacterium and Lactobacillus. Conclusions: This study shows that particular isolates of Blastocystis ST7 cause changes in microbiota populations and potentially lead to an imbalance of the gut microbiota. This study suggests that certain isolates of Blastocystis exert their pathogenic effects through disruption of the gut microbiota and provides a counterpoint to the increasing reports indicating the commensal nature of this ubiquitous parasite.
Source Title: MICROBIOME
URI: https://scholarbank.nus.edu.sg/handle/10635/155019
ISSN: 2049-2618
2049-2618
DOI: 10.1186/s40168-019-0644-3
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