Please use this identifier to cite or link to this item:
Title: Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons
Authors: Ge J. 
Zhang C.-W.
Ng X.W.
Peng B. 
Pan S. 
Du S. 
Wang D. 
Li L.
Lim K.-L. 
Wohland T. 
Yao S.Q. 
Issue Date: 2016
Publisher: Wiley-VCH Verlag
Citation: Ge J., Zhang C.-W., Ng X.W., Peng B., Pan S., Du S., Wang D., Li L., Lim K.-L., Wohland T., Yao S.Q. (2016). Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons. Angewandte Chemie - International Edition 55 (16) : 4933-4937. ScholarBank@NUS Repository.
Abstract: Newly synthesized proteins constitute an important subset of the proteome involved in every cellular process, yet existing chemical tools used to study them have major shortcomings. Herein we report a suite of cell-permeable puromycin analogues capable of being metabolically incorporated into newly synthesized proteins in different mammalian cells, including neuronal cells. Subsequent labeling with suitable bioorthogonal reporters, in both fixed and live cells, enabled direct imaging and enrichment of these proteins. By taking advantage of the mutually orthogonal reactivity of these analogues, we showed multiplexed labeling of different protein populations, as well as quantitative measurements of protein dynamics by fluorescence correlation spectroscopy, could be achieved in live-cell environments. Tag and see: A suite of cell-permeable puromycin analogues that are capable of multiplexed imaging of newly synthesized proteins in live cells and neurons has been developed. For the first time, diffusion dynamics of newly synthesized proteins inside live neuron-like dendritic cells were quantitatively measured, revealing a heterogeneous behavior. � 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Source Title: Angewandte Chemie - International Edition
ISSN: 14337851
DOI: 10.1002/anie.201511030
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.