Please use this identifier to cite or link to this item: https://doi.org/10.1002/anie.201511030
DC FieldValue
dc.titlePuromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons
dc.contributor.authorGe J.
dc.contributor.authorZhang C.-W.
dc.contributor.authorNg X.W.
dc.contributor.authorPeng B.
dc.contributor.authorPan S.
dc.contributor.authorDu S.
dc.contributor.authorWang D.
dc.contributor.authorLi L.
dc.contributor.authorLim K.-L.
dc.contributor.authorWohland T.
dc.contributor.authorYao S.Q.
dc.date.accessioned2018-12-20T08:18:34Z
dc.date.available2018-12-20T08:18:34Z
dc.date.issued2016
dc.identifier.citationGe J., Zhang C.-W., Ng X.W., Peng B., Pan S., Du S., Wang D., Li L., Lim K.-L., Wohland T., Yao S.Q. (2016). Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons. Angewandte Chemie - International Edition 55 (16) : 4933-4937. ScholarBank@NUS Repository. https://doi.org/10.1002/anie.201511030
dc.identifier.issn14337851
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/150090
dc.description.abstractNewly synthesized proteins constitute an important subset of the proteome involved in every cellular process, yet existing chemical tools used to study them have major shortcomings. Herein we report a suite of cell-permeable puromycin analogues capable of being metabolically incorporated into newly synthesized proteins in different mammalian cells, including neuronal cells. Subsequent labeling with suitable bioorthogonal reporters, in both fixed and live cells, enabled direct imaging and enrichment of these proteins. By taking advantage of the mutually orthogonal reactivity of these analogues, we showed multiplexed labeling of different protein populations, as well as quantitative measurements of protein dynamics by fluorescence correlation spectroscopy, could be achieved in live-cell environments. Tag and see: A suite of cell-permeable puromycin analogues that are capable of multiplexed imaging of newly synthesized proteins in live cells and neurons has been developed. For the first time, diffusion dynamics of newly synthesized proteins inside live neuron-like dendritic cells were quantitatively measured, revealing a heterogeneous behavior. � 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.publisherWiley-VCH Verlag
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentBIOLOGY (NU)
dc.contributor.departmentBIOMED INST FOR GLOBAL HEALTH RES & TECH
dc.description.doi10.1002/anie.201511030
dc.description.sourcetitleAngewandte Chemie - International Edition
dc.description.volume55
dc.description.issue16
dc.description.page4933-4937
dc.published.statepublished
dc.grant.fundingagencyNMRC, National Medical Research Council
dc.grant.fundingagencyMinistry of Education
dc.grant.fundingagencyMinistry of Education
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