Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.scr.2016.12.014
| Title: | Derivation of human induced pluripotent stem cell (iPSC) line with LRRK2 gene R1398H variant in Parkinson's disease | Authors: | Ma D. Tio M. Ng S.H. Li Zeng Lim C.Y.Y. Zhao Y. Tan E.K. |
Issue Date: | 2017 | Publisher: | Elsevier B.V. | Citation: | Ma D., Tio M., Ng S.H., Li Zeng, Lim C.Y.Y., Zhao Y., Tan E.K. (2017). Derivation of human induced pluripotent stem cell (iPSC) line with LRRK2 gene R1398H variant in Parkinson's disease. Stem Cell Research 18 : 48-50. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2016.12.014 | Abstract: | Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 72-year old female Parkinson's disease (PD) patient with R1398H variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model provides a good platform for studying the mechanism of PD, and also for drug testing and gene therapy studies. © 2016 The Authors | Source Title: | Stem Cell Research | URI: | http://scholarbank.nus.edu.sg/handle/10635/148965 | ISSN: | 18735061 | DOI: | 10.1016/j.scr.2016.12.014 |
| Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
| File | Description | Size | Format | Access Settings | Version | |
|---|---|---|---|---|---|---|
| j.scr.2016.12.014.pdf | 614.75 kB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.