Please use this identifier to cite or link to this item:
|Title:||Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria||Authors:||Ho Kwok, Ki
|Issue Date:||1994||Citation:||Ho Kwok, Ki, Tan, Slan (1994). Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria. Journal of Liquid Chromatography 17 (4) : 833-845. ScholarBank@NUS Repository.||Abstract:||Sphacryl S-500, a gel-filtration matrix, adsorbs water soluble proteins at high concentrations of ammonium sulfate. It has been used here as a matrix for adsorption chromatography, separating isoforms of cytochrome c553 and ferredoxin from Oscillatoria sp. and Microcystis aeruginosa, respectively. The isoforms of these proteins were separated at 35% and 40% saturated ammonium sulfate, respectively. Using this adsorption chromatography, we have purifield high levels of individual isoforms with improved resolution over conventional procedures such as polyacrylamide gel electrophoresis and ion-exchange chromatography.||Source Title:||Journal of Liquid Chromatography||URI:||http://scholarbank.nus.edu.sg/handle/10635/134121||ISSN:||01483919|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 8, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.