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|Title:||Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria|
|Authors:||Ho Kwok, Ki |
|Citation:||Ho Kwok, Ki, Tan, Slan (1994). Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria. Journal of Liquid Chromatography 17 (4) : 833-845. ScholarBank@NUS Repository.|
|Abstract:||Sphacryl S-500, a gel-filtration matrix, adsorbs water soluble proteins at high concentrations of ammonium sulfate. It has been used here as a matrix for adsorption chromatography, separating isoforms of cytochrome c553 and ferredoxin from Oscillatoria sp. and Microcystis aeruginosa, respectively. The isoforms of these proteins were separated at 35% and 40% saturated ammonium sulfate, respectively. Using this adsorption chromatography, we have purifield high levels of individual isoforms with improved resolution over conventional procedures such as polyacrylamide gel electrophoresis and ion-exchange chromatography.|
|Source Title:||Journal of Liquid Chromatography|
|Appears in Collections:||Staff Publications|
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