Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134121
Title: Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria
Authors: Ho Kwok, Ki 
Tan, Slan
Issue Date: 1994
Citation: Ho Kwok, Ki, Tan, Slan (1994). Use of adsorption chromatography on sephacryl S-500 for improved separation of isoforms of soluble photosynthetic catalysts from cyanobacteria. Journal of Liquid Chromatography 17 (4) : 833-845. ScholarBank@NUS Repository.
Abstract: Sphacryl S-500, a gel-filtration matrix, adsorbs water soluble proteins at high concentrations of ammonium sulfate. It has been used here as a matrix for adsorption chromatography, separating isoforms of cytochrome c553 and ferredoxin from Oscillatoria sp. and Microcystis aeruginosa, respectively. The isoforms of these proteins were separated at 35% and 40% saturated ammonium sulfate, respectively. Using this adsorption chromatography, we have purifield high levels of individual isoforms with improved resolution over conventional procedures such as polyacrylamide gel electrophoresis and ion-exchange chromatography.
Source Title: Journal of Liquid Chromatography
URI: http://scholarbank.nus.edu.sg/handle/10635/134121
ISSN: 01483919
Appears in Collections:Staff Publications

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