Please use this identifier to cite or link to this item:
Title: Subzonal transfer of multiple sperm (MIST) into early human embryos
Authors: Ng, S.C. 
Sathananthan, A.H.
Bongso, T.A. 
Ratnam, S.S. 
Tok, V.C.N.
Ho, J.K.C.
Keywords: fertilization
Issue Date: 1990
Citation: Ng, S.C., Sathananthan, A.H., Bongso, T.A., Ratnam, S.S., Tok, V.C.N., Ho, J.K.C. (1990). Subzonal transfer of multiple sperm (MIST) into early human embryos. Molecular Reproduction and Development 26 (3) : 253-260. ScholarBank@NUS Repository.
Abstract: Microinsemination sperm transfer (MIST) is a technique whereby sperm are transferred into the perivitelline space (PVS) with the aid of a micromanipulator. MIST is now used to investigate whether blastomere membranes of early human embryos are capable of fusing with the sperm as in the metaphase II oocyte. Between 10 and 30 sperm were transferred into 11 donated human embyros between pronuclear and 16 cell stage. After culture for 6-24 hr in vitro, the embryos were fixed for transmission electron microscopy (TEM). Both acrosome-intact and acrosome-reacted sperm were located in the PVS and between blastomeres. Sperm in the PVS were sometimes penetrating the inner regions of the zona. Sperm-blastomere membrane fusion was not observed, but sperm tail incorporation by phagocytosis was occasionally evident. Sperm heads incorporated into blastomeres were often located in membrane-bound vesicles both acrosome-intact and acrosome-reacted sperm heads were lying passively in vacuoles . Acrosome-reated sperm heads were lying passively in vacuoles or were undergoing degenerative changes at their surfaces. Sperm chromatin decondensation was not observed in any of the sperm heads that were detected in the blastomers. The evidence presented clearly shows that sperm heads are incapable of expanding their chromatin to form typical male pronuclei following MIST into early human embryos.
Source Title: Molecular Reproduction and Development
ISSN: 1040452X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 8, 2018


checked on Dec 31, 2018

Page view(s)

checked on Nov 8, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.