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Title: Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay
Authors: Simpson, P.R.
Yu, X.H.
Redza, Z.M.
Anson, J.G.
Chan, S.H. 
Lin, Y.
Keywords: DNA extraction
Issue Date: Dec-1997
Citation: Simpson, P.R., Yu, X.H., Redza, Z.M., Anson, J.G., Chan, S.H., Lin, Y. (1997-12). Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay. Journal of Virological Methods 69 (1-2) : 197-208. ScholarBank@NUS Repository.
Abstract: A rapid assay for the quantification of hepatitis B virus DNA in human serum was developed. The principle of the method combines competitive polymerase chain reaction (cPCR) (for the controlled amplification of hepatitis B virus DNA) and scintillation proximity assay (SPA) technology (for rapid detection and quantitation of PCR products). It also incorporates a reproducible and simple method for the preparation of serum DNA suitable for PCR amplification. The assay has a better linear dynamic range than traditional methods that use 32P to detect PCR products. It was applied to a range of hepatitis B virus (HBV) surface antigen positive (HBsAg+) sera, and shown to be more sensitive than a commercially available HBV DNA kit.
Source Title: Journal of Virological Methods
ISSN: 01660934
DOI: 10.1016/S0166-0934(97)00159-6
Appears in Collections:Staff Publications

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