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|Title:||Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay||Authors:||Simpson, P.R.
|Issue Date:||Dec-1997||Citation:||Simpson, P.R., Yu, X.H., Redza, Z.M., Anson, J.G., Chan, S.H., Lin, Y. (1997-12). Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay. Journal of Virological Methods 69 (1-2) : 197-208. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(97)00159-6||Abstract:||A rapid assay for the quantification of hepatitis B virus DNA in human serum was developed. The principle of the method combines competitive polymerase chain reaction (cPCR) (for the controlled amplification of hepatitis B virus DNA) and scintillation proximity assay (SPA) technology (for rapid detection and quantitation of PCR products). It also incorporates a reproducible and simple method for the preparation of serum DNA suitable for PCR amplification. The assay has a better linear dynamic range than traditional methods that use 32P to detect PCR products. It was applied to a range of hepatitis B virus (HBV) surface antigen positive (HBsAg+) sera, and shown to be more sensitive than a commercially available HBV DNA kit.||Source Title:||Journal of Virological Methods||URI:||http://scholarbank.nus.edu.sg/handle/10635/133656||ISSN:||01660934||DOI:||10.1016/S0166-0934(97)00159-6|
|Appears in Collections:||Staff Publications|
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