Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0166-0934(97)00159-6
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dc.titleQuantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay
dc.contributor.authorSimpson, P.R.
dc.contributor.authorYu, X.H.
dc.contributor.authorRedza, Z.M.
dc.contributor.authorAnson, J.G.
dc.contributor.authorChan, S.H.
dc.contributor.authorLin, Y.
dc.date.accessioned2016-12-20T08:38:34Z
dc.date.available2016-12-20T08:38:34Z
dc.date.issued1997-12
dc.identifier.citationSimpson, P.R., Yu, X.H., Redza, Z.M., Anson, J.G., Chan, S.H., Lin, Y. (1997-12). Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay. Journal of Virological Methods 69 (1-2) : 197-208. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(97)00159-6
dc.identifier.issn01660934
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/133656
dc.description.abstractA rapid assay for the quantification of hepatitis B virus DNA in human serum was developed. The principle of the method combines competitive polymerase chain reaction (cPCR) (for the controlled amplification of hepatitis B virus DNA) and scintillation proximity assay (SPA) technology (for rapid detection and quantitation of PCR products). It also incorporates a reproducible and simple method for the preparation of serum DNA suitable for PCR amplification. The assay has a better linear dynamic range than traditional methods that use 32P to detect PCR products. It was applied to a range of hepatitis B virus (HBV) surface antigen positive (HBsAg+) sera, and shown to be more sensitive than a commercially available HBV DNA kit.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0166-0934(97)00159-6
dc.subjectDNA extraction
dc.subjectHBV DNA
dc.subjectSensitivity
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/S0166-0934(97)00159-6
dc.description.sourcetitleJournal of Virological Methods
dc.description.volume69
dc.description.issue1-2
dc.description.page197-208
dc.description.codenJVMED
dc.identifier.isiut000071972600021
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