Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S0166-0934(97)00159-6
DC Field | Value | |
---|---|---|
dc.title | Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay | |
dc.contributor.author | Simpson, P.R. | |
dc.contributor.author | Yu, X.H. | |
dc.contributor.author | Redza, Z.M. | |
dc.contributor.author | Anson, J.G. | |
dc.contributor.author | Chan, S.H. | |
dc.contributor.author | Lin, Y. | |
dc.date.accessioned | 2016-12-20T08:38:34Z | |
dc.date.available | 2016-12-20T08:38:34Z | |
dc.date.issued | 1997-12 | |
dc.identifier.citation | Simpson, P.R., Yu, X.H., Redza, Z.M., Anson, J.G., Chan, S.H., Lin, Y. (1997-12). Quantification of hepatitis B virus DNA using competitive PCR and a scintillation proximity assay. Journal of Virological Methods 69 (1-2) : 197-208. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(97)00159-6 | |
dc.identifier.issn | 01660934 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/133656 | |
dc.description.abstract | A rapid assay for the quantification of hepatitis B virus DNA in human serum was developed. The principle of the method combines competitive polymerase chain reaction (cPCR) (for the controlled amplification of hepatitis B virus DNA) and scintillation proximity assay (SPA) technology (for rapid detection and quantitation of PCR products). It also incorporates a reproducible and simple method for the preparation of serum DNA suitable for PCR amplification. The assay has a better linear dynamic range than traditional methods that use 32P to detect PCR products. It was applied to a range of hepatitis B virus (HBV) surface antigen positive (HBsAg+) sera, and shown to be more sensitive than a commercially available HBV DNA kit. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0166-0934(97)00159-6 | |
dc.subject | DNA extraction | |
dc.subject | HBV DNA | |
dc.subject | Sensitivity | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY | |
dc.description.doi | 10.1016/S0166-0934(97)00159-6 | |
dc.description.sourcetitle | Journal of Virological Methods | |
dc.description.volume | 69 | |
dc.description.issue | 1-2 | |
dc.description.page | 197-208 | |
dc.description.coden | JVMED | |
dc.identifier.isiut | 000071972600021 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.